caa a22 4500 477100171 CHVBK 20180405111538.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF00200297 doi (NATIONALLICENCE)springer-10.1007/BF00200297 Changes in the cellular localization of cytosolic glutamine synthetase protein in vascular bundles of rice leaves at various stages of development [Elektronische Daten] [Nozomu Sakurai, Toshihiko Hayakawa, Teiji Nakamura, Tomoyuki Yamaya] Cellular localization of cytosolic glutamine synthetase (GS1; EC 6.3.1.2) in vascular bundles of leaf blades of rice (Oryza sativa L.), at the stage at which leaf blades 6 (the lowest position) to 10 were fully expanded, was investigated immunocytologically with an affinity-purified anti-GS1 immunoglobulin G. Strong signals for GS1 protein were detected in companion cells of large vascular bundles when blades 6-8 were tested. Signals for GS1 were also observed in vascular-parenchyma cells of both large and small vascular bundles. The results further support our hypothesis that GS1 is important for the export of leaf nitrogen from senescing leaves. The signals in companion cells were less striking in the younger green leaves and were hardly detected in the non-green portion of the 11th blade. In the non-green blades, strong signals for GS1 protein were detected in sclerenchyma and xylemparenchyma cells. When total GS extracts prepared from the 6th,10th, and the non-green 11th blades were subjected to anion-exchange chromatography, the activity of GS1 was clearly separated from that of chloroplastic GS, indicating that GS1 proteins detected in the vascular tissues were able to synthesize glutamine. The function of GS1 detected in the developing leaves is discussed. Springer-Verlag, 1996 Enzyme localization nationallicence Glutamine synthetase nationallicence Nitrogen remobilization nationallicence Oryza nationallicence Vascular bundle nationallicence Fd-GOGAT : ferredoxin-dependent glutamate synthase nationallicence GS1 : cytosolic glutamine synthetase nationallicence GS2 : plastidic glutamine synthetase nationallicence IgG : immunoglobulin G nationallicence Sakurai Nozomu Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981, Sendai, Japan aut Hayakawa Toshihiko Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981, Sendai, Japan aut Nakamura Teiji Department of Applied Bio-Sciences, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981, Sendai, Japan aut Yamaya Tomoyuki Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981, Sendai, Japan aut Planta Springer-Verlag 200/3(1996-11-01), 306-311 0032-0935 200:3<306 1996 200 425 https://doi.org/10.1007/BF00200297 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00200297 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakurai Nozomu Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981, Sendai, Japan aut NATIONALLICENCE 700 1- Hayakawa Toshihiko Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981, Sendai, Japan aut NATIONALLICENCE 700 1- Nakamura Teiji Department of Applied Bio-Sciences, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981, Sendai, Japan aut NATIONALLICENCE 700 1- Yamaya Tomoyuki Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981, Sendai, Japan aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/3(1996-11-01), 306-311 0032-0935 200:3<306 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer