caa a22 4500 477100198 CHVBK 20180405111538.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00231392 doi (NATIONALLICENCE)springer-10.1007/BF00231392 Processing in vitro of pronapin, the 2S storage-protein precursor of Brassica napus produced in a baculovirus expression system [Elektronische Daten] [Eva Muren, Lars Rask] The maturation of the 2S albumin, napin, in Brassica napus L. involves removal of an amino-terminal and an internal propeptide. Pulse-chase experiments with B. napus embryos showed that intermediates are detectable during the pronapin processing. Intact pronapin was expressed by baculovirus in Spodoptera frugiperda insect cells in order to obtain substrate for studying the processing event. Processing of pronapin with a crude B. napus embryo protein extract resulted in several fragments of similar sizes to those of napin heavy and light chains. The character of the major processing activity in the B. napus extract suggested that it was due to an aspartic proteinase. A secondary activity indicated an additional endo-proteinase involved in the pronapin processing. Limited proteolysis of pronapin with a purified aspartic proteinase from Hordeum vulgare showed that cleavage occurred exclusively in the prosequences. The cleavage products formed in-vitro requires additional trimming of the propeptides in order to obtain the subunits of mature napin. Springer-Verlag, 1996 Brassica nationallicence Napin nationallicence Processing nationallicence Proteinase nationallicence Storage protein nationallicence 2S albumin nationallicence Muren Eva Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-75007, Uppsala, Sweden aut Rask Lars Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-75007, Uppsala, Sweden aut Planta Springer-Verlag 200/4(1996-12-01), 373-379 0032-0935 200:4<373 1996 200 425 https://doi.org/10.1007/BF00231392 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231392 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muren Eva Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-75007, Uppsala, Sweden aut NATIONALLICENCE 700 1- Rask Lars Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-75007, Uppsala, Sweden aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/4(1996-12-01), 373-379 0032-0935 200:4<373 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer