caa a22 4500 477100244 CHVBK 20180405111539.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00231391 doi (NATIONALLICENCE)springer-10.1007/BF00231391 A xylogalacturonan whose level is dependent on the size of cell clusters is present in the pectin from cultured carrot cells [Elektronische Daten] [Akira Kikuchi, Yusuke Edashige, Tadashi Ishii, Shinobu Satoh] A substantial level of xylose was detected in the pectic polysaccharides that had been extracted from carrot (Daucus carota L.) calli and purified by gel-permeation and ion-exchange chromatography. The results of the removal of neutral sugar chains and β-elimination indicated that the xylose was not included in the neutral sugar chains but was directly bound to a polygalac-turonic-acid backbone. Methylation analysis confirmed that the xylose was directly linked to galacturonic acid at position 2 or 3, as a terminal residue. The amount of xylose was positively correlated with the size of cell clusters in several lines of cultured carrot cells. Springer-Verlag, 1996 Callus nationallicence Daucus (cell walls) nationallicence Pectin nationallicence Xylogalacturonan nationallicence EC : embryogenic callus nationallicence 4-GalA : 4-linked galacturonic acid nationallicence NC : non-embryogenic callus nationallicence T-Xyl : terminal xylose nationallicence 3,4-GalA : 3,4-linked galacturonic acid nationallicence 2,4-GalA : 2,4-linked galacturonic acid nationallicence Kikuchi Akira Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut Edashige Yusuke Forestry and Forest Products Research Institute, Tsukuba, 305, Ibaraki, Japan aut Ishii Tadashi Forestry and Forest Products Research Institute, Tsukuba, 305, Ibaraki, Japan aut Satoh Shinobu Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut Planta Springer-Verlag 200/4(1996-12-01), 369-372 0032-0935 200:4<369 1996 200 425 https://doi.org/10.1007/BF00231391 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231391 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kikuchi Akira Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Edashige Yusuke Forestry and Forest Products Research Institute, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ishii Tadashi Forestry and Forest Products Research Institute, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Satoh Shinobu Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/4(1996-12-01), 369-372 0032-0935 200:4<369 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer