caa a22 4500 477100252 CHVBK 20180405111539.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00231395 doi (NATIONALLICENCE)springer-10.1007/BF00231395 Transcriptional and post-transcriptional activation of genes in salt-tolerant alfalfa cells [Elektronische Daten] [Ilga Winicov, Muthu Krishnan] Salt-tolerant cell lines of alfalfa (Medicago sativa L.) selected in this laboratory showed increased mRNA accumulation for both nuclear- and chloroplastencoded genes involved in photosynthesis as well as in several non-photosynthetic related functions. The basis for this constitutive and salt-dependent gene activation was investigated by measuring both nuclear and plastid run-on transcription from the salt-sensitive parent line and from selected salt-tolerant lines. Plastids from tolerant cells showed a 2.5-fold increase in transcription rate over those from sensitive cells and a 4.5-fold increase if isolated from tolerant cells grown in salt. Nuclei isolated from salt-tolerant cells grown on normal medium showed higher transcription of the photosynthesis-related genes rbcS, cab1 and cab4 than those from salt-sensitive cells, confirming that the salt-tolerant cells had acquired altered transcriptional regulation of these genes. However, the major salt-induced increase in steady-state mRNA accumulation, from photosynthesis-related and other genes (alfin1, pA18 and histone H3cI and H3cII genes), was not reflected in run-on assays from these same cells. These results indicated that salt-dependent post-transcriptional mRNA stabilization led to the steady-state mRNA accumulation. The mRNA stabilization appears to be transcript specific, since transcripts of a constitutively-expressed gene (Msc27) remained unaffected by growth of the tolerant cells in 171 mM NaCl. Springer-Verlag, 1996 Medicago (salt tolerance) nationallicence Photosynthesis nationallicence Post-transcriptional regulation nationallicence Salt tolerance nationallicence Gene transcription nationallicence rbcL : gene for Rubisco large subunit nationallicence rbcS : gene for Rubisco small subunit nationallicence cab-1 and cab-4 : genes encoding chlorophyll a/b-binding polypeptides nationallicence Rubisco : ribulose-l,5-bisphosphate carboxylase-oxygenase (EC 4.1.1.39) nationallicence Winicov Ilga Department of Microbiology, University of Nevada Reno, 89557, Reno, NV, USA aut Krishnan Muthu Department of Biochemistry, University of Nevada Reno, 89557, Reno, NV, USA aut Planta Springer-Verlag 200/4(1996-12-01), 397-404 0032-0935 200:4<397 1996 200 425 https://doi.org/10.1007/BF00231395 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231395 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Winicov Ilga Department of Microbiology, University of Nevada Reno, 89557, Reno, NV, USA aut NATIONALLICENCE 700 1- Krishnan Muthu Department of Biochemistry, University of Nevada Reno, 89557, Reno, NV, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/4(1996-12-01), 397-404 0032-0935 200:4<397 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer