caa a22 4500 477100260 CHVBK 20180405111539.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00231398 doi (NATIONALLICENCE)springer-10.1007/BF00231398 Detoxification of xenobiotics by plant cells: characterisation of vacuolar amphiphilic organic anion transporters [Elektronische Daten] [Mechteld Blake-Kalff, Julian Coleman] The transport activities of two primary ATP-dependent organic-anion transporters in the tonoplast of isolated barley (Hordeum vulgare L. cv. Klaxon) vacuoles have been characterised with N-ethylmaleimide glutathione (NEM-SG) and taurocholate as substrates. The transporters showed different sensitivities to organic anions and a variety of transport inhibitors and drugs. The vacuolar uptake of NEM-SG was inhibited by carbonylcyanide 4-trifluoromethoxyphenylhydrazone, 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS), S-(2,4-dinitrophenyl)glutathione, alkyl-S-glutathione derivatives and taurocholate but stimulated by probenecid. The uptake of taurocholate was inhibited by vinblastine, DIDS and probenecid. Both transporters were unaffected by verapamil. The kinetic properties of the transporters indicate a general preference for amphiphilic anions with some substrate overlap. These characteristics of the transporters are similar to those displayed by the multidrug resistance protein of mammalian drug-resistant cells. We suggest that these vacuolar transporters be described as plant multispecific organic anion transporters (pMOATs). Springer-Verlag, 1996 Glutathione S-conjugates nationallicence Hordeum (organic-anion transporters) nationallicence Membrane transport nationallicence Taurocholate nationallicence Vacuole nationallicence Xenobiotic nationallicence Bm-S : bimane S-glutathione nationallicence DIDS : 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid nationallicence DNP-SG : S-(2,4-dinitrophenyl)glutathione nationallicence FCCP : carbonylcyanide 4-trifluoromethoxyphenylhydrazone nationallicence LTC4 : cysteinyl leukotriene nationallicence MDR : multidrug transporter nationallicence MRP : multidrug resistance protein nationallicence NEM-SG : N-ethylmaleimide glutathione nationallicence Blake-Kalff Mechteld Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK aut Coleman Julian Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK aut Planta Springer-Verlag 200/4(1996-12-01), 426-431 0032-0935 200:4<426 1996 200 425 https://doi.org/10.1007/BF00231398 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231398 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blake-Kalff Mechteld Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK aut NATIONALLICENCE 700 1- Coleman Julian Biochemistry and Physiology Department, IACR-Rothamsted, ALS 2JQ, Harpenden, Herts., UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/4(1996-12-01), 426-431 0032-0935 200:4<426 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer