caa a22 4500 477100287 CHVBK 20180405111539.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00208304 doi (NATIONALLICENCE)springer-10.1007/BF00208304 Feed-back regulation of gibberellin biosynthesis and gene expression in Pisum sativum L [Elektronische Daten] [David Martin, William Proebsting, T. Parks, William Dougherty, Theodor Lange, Mervyn Lewis, Paul Gaskin, Peter Hedden] Treatment of tall and dwarf (3β-hydroxylase impaired) genotypes of pea (Pisum sativum L.) with the synthetic, highly active gibberellin (GA), 2,2-dimethyl GA4, reduced the shoot contents of C19-GAs, including GA1, and increased the concentration of the C20-GA, GA19. In shoots of the slender (la cry s ) mutant, the content of C19-GAs was lower and GA19 content was higher than in those of the tall line. Metabolism of GA19 and GA20 in leaves of a severe (na) GA-deficient dwarf mutant was reduced by GA treatment. The results suggest feedback regulation of the 20-oxidation and 3β-hydroxylation reactions. Feed-back regulation of GA 20-oxidation was studied further using a cloned GA 20-oxidase cDNA from pea. The cDNA, Ps074, was isolated using polymerase chain reaction with degenerate oligonucleotide primers based on pumpkin and Arabidopsis 20-oxidase sequences. After expression of this cDNA clone in Escherichia coli, the product oxidized GA12 to GA15, GA24 and the C19-GA, GA9, which was the major product. The 13-hydroxylated substrate GA53 was similarly oxidized, but less effectively than GA12, giving mainly GA44 with low yields of GA19 and GA20. Ps074 hybridized to polyadenylated RNA from expanding shoots of pea. Amounts of this transcript were less in the slender genotype than in the tall line and were reduced in GA-deficient genotypes by treatment with GA3, suggesting that there is feed-back regulation of GA 20-oxidase gene expression. Springer-Verlag, 1996 Gibberellin biosynthesis (regulation) nationallicence Gibberellin 20-oxidase (cDNA cloning) nationallicence Pisum nationallicence GA : gibberellin nationallicence GC-SIM : gas chromatography-single ion monitoring nationallicence IPTG : isopropyl-β-d-thiogalactoside nationallicence PCR : polymerase chain reaction nationallicence Martin David Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, 97331-7304, Corvallis, OR, USA aut Proebsting William Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, 97331-7304, Corvallis, OR, USA aut Parks T. Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, 97331-7304, Corvallis, OR, USA aut Dougherty William Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, 97331-7304, Corvallis, OR, USA aut Lange Theodor Pflanzenphysiologisches Institut und Botanischer Garten der Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany aut Lewis Mervyn IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, BS18 9AF, Bristol, UK aut Gaskin Paul IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, BS18 9AF, Bristol, UK aut Hedden Peter IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, BS18 9AF, Bristol, UK aut Planta Springer-Verlag 200/2(1996-10-01), 159-166 0032-0935 200:2<159 1996 200 425 https://doi.org/10.1007/BF00208304 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00208304 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martin David Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, 97331-7304, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Proebsting William Department of Horticulture and Center for Gene Research and Biotechnology, Oregon State University, 97331-7304, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Parks T. Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, 97331-7304, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Dougherty William Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, 97331-7304, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Lange Theodor Pflanzenphysiologisches Institut und Botanischer Garten der Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany aut NATIONALLICENCE 700 1- Lewis Mervyn IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, BS18 9AF, Bristol, UK aut NATIONALLICENCE 700 1- Gaskin Paul IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, BS18 9AF, Bristol, UK aut NATIONALLICENCE 700 1- Hedden Peter IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, BS18 9AF, Bristol, UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/2(1996-10-01), 159-166 0032-0935 200:2<159 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer