caa a22 4500 477100295 CHVBK 20180405111539.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00208315 doi (NATIONALLICENCE)springer-10.1007/BF00208315 The raz1 mutant of Arabidopsis thaliana lacks the activity of a high-affinity amino acid transporter [Elektronische Daten] [N. Verbruggen, A. Borstlap, M. Jacobs, M. Van Montagu, E. Messens] The raz1 mutant of Arabidopsis thaliana (L.) Heynh. has been selected as resistant to the toxic proline analogue, azetidine-2-carboxylic acid (2AZ). Seedlings of the mutant tolerated fivefold higher concentrations of 2AZ (ED50 = 0.25 mM) than the wild-type seedlings (ED50 = 0.05 mM). The mutant gene was found to be semi-dominant and the corresponding RAZ1 locus was mapped on chromosome 5 at 69.6±1.8 cM. The resistance to 2AZ could be fully and exclusively accounted for by the lower uptake rate of the proline analogue in the mutant. The influx of L-proline in roots of wild-type seedlings could be dissected into two components: (i) a component with a high affinity and a low capacity for l-proline (K m≈20 gmM, V max≈60 nmol·(g FW)-1·h-1) and also a high affinity for L-2AZ (K i≈40 μM) and (ii) a low-affinity, high-capacity component (K m≈5 mM: V max = 1300 nmol·(g FW)-1·h-1). Clearly, the raz1 mutation affects the activity of a high-affinity transporter, because the high-affinity uptake of proline in the mutant was at least fivefold lower than in the wild-type, whereas the low-affinity uptake was unchanged. Springer-Verlag, 1996 Arabidopsis nationallicence Amino acid transport systems nationallicence Gene mapping nationallicence Proline (uptake kinetics) nationallicence 2AZ : azetidine-2-carboxylic acid nationallicence Verbruggen N. Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut Borstlap A. Vakgroep Botanische Oecologie en Evolutiebiologie, Universiteit Utrecht, NL-3584 CA, Utrecht, The Netherlands aut Jacobs M. Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, B-1640, Sint-Genesius-Rode, Belgium aut Van Montagu M. Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut Messens E. Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut Planta Springer-Verlag 200/2(1996-10-01), 247-253 0032-0935 200:2<247 1996 200 425 https://doi.org/10.1007/BF00208315 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00208315 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Verbruggen N. Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Borstlap A. Vakgroep Botanische Oecologie en Evolutiebiologie, Universiteit Utrecht, NL-3584 CA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Jacobs M. Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, B-1640, Sint-Genesius-Rode, Belgium aut NATIONALLICENCE 700 1- Van Montagu M. Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- Messens E. Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/2(1996-10-01), 247-253 0032-0935 200:2<247 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer