caa a22 4500 477100333 CHVBK 20180405111539.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00208308 doi (NATIONALLICENCE)springer-10.1007/BF00208308 Characterisation of a glutathione reductase gene and its genetic locus from pea ( Pisum sativum L.) [Elektronische Daten] [Philip Mullineaux, Corine Enard, Roger Hellens, Gary Creissen] A cDNA encoding the chloroplast/mitochondrial form of glutathione reductase (GR; EC 1.6.4.2) from pea (Pisum sativum L.) was used to map a single GR locus, named GOR1. In two domesticated genotypes of pea (cv. Birte and JI 399) it is likely that the GOR1 locus contains a single gene. However, in a semi-domesticated land race of pea (JI 281) two distinct but closely related sets of GR gene sequences were detected at the GOR1 locus. The extra GR sequences in JI 281 represent either a second intact gene or a partial or pseudogene copy. A GR gene was cloned from cv. Birte, sequenced and its structure analysed. No feature of the transcription or structure of the gene suggested a mechanism for generating any more than one form of GR. From these data plus previously published biochemical evidence we suggest that a second, distinct gene encoding for the cytosolic form of GR should be present in peas. The GOR1-encoded GR mRNA can be detected in all main organs of the plant and no alternative spliced species was present which could perhaps account for the generation of multiple isoforms of GR. The mismatch between the number of charge-separable isoforms in pea and the proposed number of genes suggests that different GR isoforms arise by some form of post-translational modification. Springer-Verlag, 1996 Gene structure nationallicence Genetic locus nationallicence Gene transcripts nationallicence Glutathione reductase (isoforms) nationallicence Pisum nationallicence GR : glutathione reductase nationallicence PCR : polymerase chain reaction nationallicence RACE : rapid amplification of cDNA ends nationallicence RFLP : restriction fragment length polymorphism nationallicence Mullineaux Philip John Innes Centre, Norwich Research Park, NR47UH, Colney, Norwich, UK aut Enard Corine John Innes Centre, Norwich Research Park, NR47UH, Colney, Norwich, UK aut Hellens Roger John Innes Centre, Norwich Research Park, NR47UH, Colney, Norwich, UK aut Creissen Gary John Innes Centre, Norwich Research Park, NR47UH, Colney, Norwich, UK aut Planta Springer-Verlag 200/2(1996-10-01), 186-194 0032-0935 200:2<186 1996 200 425 https://doi.org/10.1007/BF00208308 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00208308 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mullineaux Philip John Innes Centre, Norwich Research Park, NR47UH, Colney, Norwich, UK aut NATIONALLICENCE 700 1- Enard Corine John Innes Centre, Norwich Research Park, NR47UH, Colney, Norwich, UK aut NATIONALLICENCE 700 1- Hellens Roger John Innes Centre, Norwich Research Park, NR47UH, Colney, Norwich, UK aut NATIONALLICENCE 700 1- Creissen Gary John Innes Centre, Norwich Research Park, NR47UH, Colney, Norwich, UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/2(1996-10-01), 186-194 0032-0935 200:2<186 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer