caa a22 4500 477100449 CHVBK 20180405111539.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF00208305 doi (NATIONALLICENCE)springer-10.1007/BF00208305 Expression of the JIM8 cell wall epitope in carrot somatic embryogenesis [Elektronische Daten] [Marcel Toonen, Ed Schmidt, Theo Hendriks, Harrie Verhoeven, Ab van Kammen, Sacco de Vries] Certain single cells in carrot (Daucus carota L.) suspension cultures react with the monoclonal antibody JIM8, and it has been proposed that these cells represent a transitional stage in somatic embryo formation. Shortly after isolation of the single cells by sieving, up to 80% of the cells react with JIM8. Within 4 d, JIM8 labelling becomes restricted to 1% of the single cells. To obtain evidence for the proposed correlation between expression of the JIM8 cell wall epitope and somatic embryo formation the developmental fate of carrot single cells labelled with JIM8 was determined by cell tracking. The results, obtained by recording 43 000 cells, show that only few JIM8-labelled cells give rise to embryos, and most somatic embryos develop from cells devoid of the JIM8 cell wall epitope. We therefore conclude that the presence of the JIM8 cell wall epitope does not coincide with the ability of single suspension cells to form embryos. Springer-Verlag, 1996 Arabinogalactan protein epitope nationallicence Cell tracking nationallicence Daucus nationallicence Monoclonal antibody (JIM8) nationallicence Somatic embryogenesis nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence AGP : arabino galactan protein nationallicence B5-0 : Gamborg's B5 medium nationallicence B5-0.2 : Gamborg's B5 medium supplemented with 0.2 μM 2,4-D nationallicence FITC : fluoresceïn isothiocyanate nationallicence PBS : phosphate-buffered saline nationallicence Toonen Marcel Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut Schmidt Ed Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut Hendriks Theo Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut Verhoeven Harrie Centre of Plant Breeding and Reproduction Research (CPRO-DLO), Droevendaalsesteeg 1, P.O. Box 16, NL-6700 AA, Wageningen, The Netherlands aut van Kammen Ab Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut de Vries Sacco Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut Planta Springer-Verlag 200/2(1996-10-01), 167-173 0032-0935 200:2<167 1996 200 425 https://doi.org/10.1007/BF00208305 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00208305 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toonen Marcel Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Schmidt Ed Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Hendriks Theo Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Verhoeven Harrie Centre of Plant Breeding and Reproduction Research (CPRO-DLO), Droevendaalsesteeg 1, P.O. Box 16, NL-6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van Kammen Ab Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- de Vries Sacco Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Planta Springer-Verlag 200/2(1996-10-01), 167-173 0032-0935 200:2<167 1996 200 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer