caa a22 4500 47710052X CHVBK 20180405111539.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00197590 doi (NATIONALLICENCE)springer-10.1007/BF00197590 Intracellular magnetophoresis of amyloplasts and induction of root curvature [Elektronische Daten] [Oleg Kuznetsov, Karl Hasenstein] High-gradient magnetic fields (HGMFs) were used to induce intracellular magnetophoresis of amyloplasts. The HGMFs were generated by placing a small ferromagnetic wedge into a uniform magnetic field or at the gap edge between two permanent magnets. In the vicinity of the tip of the wedge the dynamic factor of the magnetic field, ▽(H2/2), was about 109 Oe2 · cm−1, which subjected the amyloplasts to a force comparable to that of gravity. When roots of 2-d-old seedlings of flax (Linum usitatissimum L.) were positioned vertically and exposed to an HGMF, curvature away from the wedge was transient and lasted approximately 1 h. Average curvature obtained after placing magnets, wedge and seedlings on a 1-rpm clinostat for 2 h was 33 ± 5 degrees. Roots of horizontally placed control seedlings without rotation curved about 47 ± 4 degrees. The time course of curvature and changes in growth rate were similar for gravicurvature and for root curvature induced by HGMFs. Microscopy showed displacement of amyloplasts in vitro and in vivo. Studies with Arabidopsis thaliana (L.) Heynh. showed that the wild type responded to HGMFs but the starchless mutant TC7 did not. The data indicate that a magnetic force can be used to study the gravisensing and response system of roots. Springer-Verlag, 1996 Arabidopsis nationallicence Gravitropism nationallicence High-gradient magnetic field nationallicence Linum nationallicence Root growth nationallicence HGMF : high-gradient magnetic field nationallicence emu : electromagnetic units nationallicence Oe : Oersted nationallicence Kuznetsov Oleg Department of Biology, University of Southwestern Louisiana, 70504-2451, Lafayette, LA, USA aut Hasenstein Karl Department of Biology, University of Southwestern Louisiana, 70504-2451, Lafayette, LA, USA aut Planta Springer-Verlag 198/1(1996-01-01), 87-94 0032-0935 198:1<87 1996 198 425 https://doi.org/10.1007/BF00197590 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197590 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuznetsov Oleg Department of Biology, University of Southwestern Louisiana, 70504-2451, Lafayette, LA, USA aut NATIONALLICENCE 700 1- Hasenstein Karl Department of Biology, University of Southwestern Louisiana, 70504-2451, Lafayette, LA, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/1(1996-01-01), 87-94 0032-0935 198:1<87 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer