caa a22 4500 477100538 CHVBK 20180405111539.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00197589 doi (NATIONALLICENCE)springer-10.1007/BF00197589 Gibberellins regulate the abundance of RNAs with sequence similarity to proteinase inhibitors, dioxygenases and dehydrogenases [Elektronische Daten] [Steven Jacobsen, Neil Olszewski] In an effort to understand the molecular mechanism of gibberellin (GA) action, we have cloned and performed an initial characterization of three cDNAs (GAD1, 2, and 3) which correspond to RNAs that become less abundant by 2 h after treatment of tomato (Lycopersicon esculentum Mill.) shoot tissue with gibberellic acid (GA3). Treatment with either auxin or ethephon also decreases the abundance of all three of the GAD RNAs. The tomato ethylene-insensitive mutant, Nr, and the GA-deficient mutant, gib1, were used to show that GA or auxin regulation of GAD RNA abundance is not dependent on ethylene sensitivity, and that ethylene or auxin regulation is not dependent on normal levels of gibberellin biosynthesis. Treatment with abscisic acid (ABA) antagonizes the GA induced suppression of the GAD1 and GAD2 RNAs. GAD1 is similar to type-II wound-inducible plant proteinase inhibitors. Like the well-characterized proteinase inhibitor II (pin II) of tomato, the GAD1 and GAD2 RNAs are wound inducible. Induction of pin II and GAD1 RNA in gib1 was found to require less-severe wounding than was required using wild-type plants or plants doubly mutant for gib1 and sit (the sit mutation causes ABA deficiency). The predicted GAD2 protein sequence is similar to 2-oxoglutarate-dependent dioxygenases while the predicted GAD3 protein sequence is similar to proteins belonging to the nonmetalloshort-chain alcohol-dehydrogenase family, especially the T ASSELSEED2 (TS2) gene of maize and bacterial hydroxysteroid dehydrogenases. Springer-Verlag, 1996 Dehydrogenase nationallicence Dioxygenase nationallicence Gene expression nationallicence Gibberellin nationallicence Lycopersicon nationallicence Proteinase inhibitor nationallicence ABA : abscisic acid nationallicence 2,4-D : 2,4-dichlorophenoxy-acetic acid nationallicence GA : gibberellin nationallicence GA3 : gibberellic acid nationallicence pin II : proteinase inhibitor II nationallicence Jacobsen Steven Department of Plant Biology and Plant Molecular Genetics Institute, University of Minnesota, 55108, St. Paul, MN, USA aut Olszewski Neil Department of Plant Biology and Plant Molecular Genetics Institute, University of Minnesota, 55108, St. Paul, MN, USA aut Planta Springer-Verlag 198/1(1996-01-01), 78-86 0032-0935 198:1<78 1996 198 425 https://doi.org/10.1007/BF00197589 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197589 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jacobsen Steven Department of Plant Biology and Plant Molecular Genetics Institute, University of Minnesota, 55108, St. Paul, MN, USA aut NATIONALLICENCE 700 1- Olszewski Neil Department of Plant Biology and Plant Molecular Genetics Institute, University of Minnesota, 55108, St. Paul, MN, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/1(1996-01-01), 78-86 0032-0935 198:1<78 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer