caa a22 4500 477100546 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00197594 doi (NATIONALLICENCE)springer-10.1007/BF00197594 Rapid reactions of spruce cells to elicitors released from the ectomycorrhizal fungus Hebeloma crustuliniforme , and inactivation of these elicitors by extracellular spruce cell enzymes [Elektronische Daten] [Peter Salzer, Gerhard Hebe, Andreas Reith, Barbara Zitterell-Haid, Harald Stransky, Katja Gaschler, Achim Hager] Elicitors released from hyphae or cell walls of the ectomycorrhizal fungus Hebeloma crustuliniforme (Bull. ex Fries.) Quél. induced in suspension-cultured cells of Picea abies (L.) Karst. a set of fast reactions: (i) an immediate efflux of Cl− into the medium, followed by a K+ efflux; (ii) an influx of Ca2+ (measured as accumulation of 45Ca2+ in the cells); (iii) a phosphorylation of a 63-kDa protein and dephosphorylation of a 65-kDa protein (detectable by 4 min after elicitor application); (iv) an alkalinization of the medium, and (v) a transient synthesis of H2O2. The removal of extracellular Ca2+ by EGTA delayed the elicitor-induced alkalinization. A further reduction of this response could be achieved by TMB-8 an inhibitor of Ca2+ release from intracellular stores. Moreover, the inhibition of protein kinase activity by staurosporine prevented the extracellular alkalinization completely. However, the effectiveness of the elicitors in inducing the extracellular alkalinization was strongly impaired by constitutively secreted enzymes of spruce cells which cleaved the elicitors to inactive fragments. It is suggested that in ectomycorrhizae the efficacy of elicitors released from fungal cell walls is controlled by apoplastic enzymes of the host; the plant itself is able to reduce the activity of fungal elicitors on their way through the plant cell wall. But those elicitors which finally reach the plasma membrane of host cells induce reactions that are similar to the early defense reactions in plant-pathogen interactions. Springer-Verlag, 1996 Ectomycorrhiza nationallicence Elicitor inactivation nationallicence Elicitor-induced reaction nationallicence Hebeloma — Picea cells nationallicence Signal transduction nationallicence DW : dry weight nationallicence FW : fresh weight nationallicence TMB-8 : 3,4,5 trimethoxybenzoic acid 8-(diethylamino)-octyl ester nationallicence Salzer Peter Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Hebe Gerhard Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Reith Andreas Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Zitterell-Haid Barbara Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Stransky Harald Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Gaschler Katja Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Hager Achim Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Planta Springer-Verlag 198/1(1996-01-01), 118-126 0032-0935 198:1<118 1996 198 425 https://doi.org/10.1007/BF00197594 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197594 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salzer Peter Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Hebe Gerhard Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Reith Andreas Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Zitterell-Haid Barbara Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Stransky Harald Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Gaschler Katja Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Hager Achim Botanisches Institut, Allgemeine Botanik and Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/1(1996-01-01), 118-126 0032-0935 198:1<118 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer