caa a22 4500 477100554 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00197586 doi (NATIONALLICENCE)springer-10.1007/BF00197586 Sugar uptake by the dermal transfer cells of developing cotyledons of Vicia faba L [Elektronische Daten] Experimental systems and general transport properties [R. McDonald, S. Fieuw, J. Patrick] Two experimental systems were developed to study the uptake of sucrose by the dermal transfer cells of developing cotyledons of Vicia faba L. First, the in-vivo state was approximated by short-term (10 min) incubation of whole cotyledons in [14C]sucrose solutions. Under these conditions, a minimum of 67% of the 14C label entered the dermal transfer cell complex. Of this, at least 40% crossed the plasma membranes of the epidermal transfer cells. Second, a protocol was developed to enzymatically isolate and purify dermal transfer cell protoplasts. The yields of the transfer cell protoplasts were relatively low and their preparation incurred a significant loss of plasma membrane. However, the protoplasts remained viable up to 24 h following purification and proved to be a suitable system to verify transport properties observed with whole cotyledons. Using these two experimental systems, it was established that [14C]sucrose uptake by the dermal transfer cells exhibited features consistent with mediated energy-dependent transport. This included saturation kinetics, competition for uptake between structurally similar molecules, and inhibition of uptake by p-chloromercuribenzenesulfonic acid and several other metabolic inhibitors. For comparative purposes, sugar uptake by the storage parenchyma of the Vicia cotyledons was also examined. In contrast to the dermal transfer cell complex, sucrose uptake by the storage parenchyma displayed characteristics consistent with simple diffusion. Springer-Verlag, 1996 Cotyledon (developing) nationallicence Protoplast (dermal transfer cells) nationallicence Sugar transport nationallicence Transfer cell (dermal) nationallicence Vicia (sugar transport) nationallicence CCCP : carbonylcyanide m-chlorophenylhydrazone nationallicence DNP : 2,4-dinitrophenol nationallicence NEM : N-ethylmaleimide nationallicence PCMBS : p-chloromercuribenzenesulfonic acid nationallicence McDonald R. Department of Biological Sciences, The University of Newcastle, 2308, NSW, Australia aut Fieuw S. Department of Biological Sciences, The University of Newcastle, 2308, NSW, Australia aut Patrick J. Department of Biological Sciences, The University of Newcastle, 2308, NSW, Australia aut Planta Springer-Verlag 198/1(1996-01-01), 54-63 0032-0935 198:1<54 1996 198 425 https://doi.org/10.1007/BF00197586 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197586 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McDonald R. Department of Biological Sciences, The University of Newcastle, 2308, NSW, Australia aut NATIONALLICENCE 700 1- Fieuw S. Department of Biological Sciences, The University of Newcastle, 2308, NSW, Australia aut NATIONALLICENCE 700 1- Patrick J. Department of Biological Sciences, The University of Newcastle, 2308, NSW, Australia aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/1(1996-01-01), 54-63 0032-0935 198:1<54 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 477100554 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer