caa a22 4500 477100570 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00197591 doi (NATIONALLICENCE)springer-10.1007/BF00197591 Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons [Elektronische Daten] [David Robinson, Hans-Peter Haschke, Giselbert Hinz, Birgit Hoh, Masayoshi Maeshima, Francis Marty] The tonoplast is usually characterized by the presence of two electrogenic proton pumps: a vacuolartype H+-ATPase and a pyrophosphatase, as well as a putative water-channel-forming protein (γ-TIP). Using a post-embedding immunogold labelling technique, we have detected the presence of these transport-protein complexes not only in the tonoplast, but also in the plasma membrane and trans Golgi elements of maturing pea (Pisum sativum L.) cotyledons. These ultrastructural observations are supported by Western blotting with highly purified plasma-membrane fractions. In contrast to the vacuolar-type H+-ATPase, whose activity was not measurable, considerable pyrophosphatase activity was detected in the plasma-membrane fraction. These results are discussed in terms of a possible temporary repository for tonoplast proteins en route to the vacuole. Springer-Verlag, 1996 V-ATPase-immunocytochemistry nationallicence Pea cotyledon nationallicence Plasma membrane nationallicence Putative γ-TIP nationallicence Pyrophosphatase nationallicence Tonoplast markers nationallicence β 27 : 27-kDa tonoplast intrinsic protein, a putative γ-TIP nationallicence PM : plasma membrane nationallicence PPase : pyrophosphatase nationallicence TIP : tonoplast intrinsic protein nationallicence TP : tonoplast nationallicence V(P)-ATPase : vacuolar (plasma membrane)-type proton-transporting ATPase nationallicence Robinson David Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany aut Haschke Hans-Peter Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Königin-Luise-Strasse 12-16a, D-14195, Berlin, Germany aut Hinz Giselbert Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany aut Hoh Birgit Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany aut Maeshima Masayoshi Laboratory of Biochemistry, School of Agricultural Sciences, Nagoya University, Japan aut Marty Francis Laboratoire de Phytobiologie Cellulaire, Université de Bourgogne, Dijon, France aut Planta Springer-Verlag 198/1(1996-01-01), 95-103 0032-0935 198:1<95 1996 198 425 https://doi.org/10.1007/BF00197591 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197591 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Robinson David Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany aut NATIONALLICENCE 700 1- Haschke Hans-Peter Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Königin-Luise-Strasse 12-16a, D-14195, Berlin, Germany aut NATIONALLICENCE 700 1- Hinz Giselbert Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany aut NATIONALLICENCE 700 1- Hoh Birgit Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-37073, Göttingen, Germany aut NATIONALLICENCE 700 1- Maeshima Masayoshi Laboratory of Biochemistry, School of Agricultural Sciences, Nagoya University, Japan aut NATIONALLICENCE 700 1- Marty Francis Laboratoire de Phytobiologie Cellulaire, Université de Bourgogne, Dijon, France aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/1(1996-01-01), 95-103 0032-0935 198:1<95 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer