caa a22 4500 477100600 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00197583 doi (NATIONALLICENCE)springer-10.1007/BF00197583 Ontogenetic regulation and photoregulation of members of the Phaseolus vulgaris L. rbcS gene family [Elektronische Daten] [Timothy Sawbridge, Marc Knight, Gareth Jenkins] The rbcS1, 2 and 3 genes of Phaseolus vulgaris are identical in coding sequence and we have studied their expression using gene-specific probes derived from their 3′ non-coding regions. The genes differ in their relative levels of expression but show only minor qualitative differences in their regulation. Transcripts of the three genes are undetectable in primary leaves in the imbibed seed, accumulate early in leaf expansion reaching a maximum 7-10 d after sowing and decrease to low levels by the time expansion is complete. Both dark-grown and light-grown primary leaves exhibit this ontogenetic pattern of expression, although the light-grown leaves have two to three times more rbcS transcripts. Light can over-ride the ontogenetic control of rbcS expression; for example, when 7-d-old dark-grown primary leaves are illuminated there is a 6- to 12-fold increase in the transcript levels of the rbcS genes. Transfer of illuminated leaves to darkness results in the loss of transcripts of all three genes, but rbcS2 transcripts persist in the dark-adapted leaves. Possible physiological mechanisms of the ontogenetic regulation of expression are discussed. Springer-Verlag, 1996 Ribulose 1,5-bisphosphate carboxylase/oxygenase nationallicence rbcS genes nationallicence Leaf development nationallicence Photoregulation nationallicence Phaseolus nationallicence rbcS : gene encoding the small subunit of Rubisco nationallicence Rubisco : ribulose 1,5-bisphosphate carboxylase/oxygenase nationallicence Sawbridge Timothy Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Bower Building, University of Glasgow, G12 8QQ, Glasgow, UK aut Knight Marc Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Bower Building, University of Glasgow, G12 8QQ, Glasgow, UK aut Jenkins Gareth Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Bower Building, University of Glasgow, G12 8QQ, Glasgow, UK aut Planta Springer-Verlag 198/1(1996-01-01), 31-38 0032-0935 198:1<31 1996 198 425 https://doi.org/10.1007/BF00197583 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197583 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sawbridge Timothy Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Bower Building, University of Glasgow, G12 8QQ, Glasgow, UK aut NATIONALLICENCE 700 1- Knight Marc Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Bower Building, University of Glasgow, G12 8QQ, Glasgow, UK aut NATIONALLICENCE 700 1- Jenkins Gareth Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Bower Building, University of Glasgow, G12 8QQ, Glasgow, UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/1(1996-01-01), 31-38 0032-0935 198:1<31 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer