caa a22 4500 477100619 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00197598 doi (NATIONALLICENCE)springer-10.1007/BF00197598 Differential redox regulation by glutathione of glutathione reductase and CuZn-superoxide dismutase gene expression in Pinus sylvestris L. needles [Elektronische Daten] [Gunnar Wingsle, Stanislaw Karpinski] Glutathione reductase (GR; EC 1.6.4.2) and superoxide dismutase (SOD; EC 1.15.1.1) are two well-known enzymes involved in the scavenging of reactive oxygen intermediates. However, little is known about the regulation of Gor and Sod genes in plant cells. To obtain information about hypothetical redox regulatory mechanisms controlling Gor and Sod gene expression we artificially enhanced the levels of reduced and oxidized forms of glutathione (GSH and GSSG) in Pinus sylvestris L. needles. Scots pine shoots were placed for 12 h in beakers containing 5 mM GSH, 5 mM GSSG or water. Increased levels of both GSSG and GSH were observed in the GSSG-treated needles after 3 h. In contrast, only the GSH level was increased by the GSH treatment. Thus, the GSH/GSSG ratio increased up to 15-fold during the GSH treatment and decreased approximately two-fold during the GSSG treatment. The GR activity was significantly higher (60%) when GSSG was applied, without any apparent change in the amount and isoform population of GR or accumulation of Gor gene transcripts. This indicates that the GR activity increased per se in the GSSG treatment. The level of cytosolic CuZn-Sod transcripts was decreased significantly by the GSH treatment without any change in enzyme activity. The chloroplastic CuZnSod gene generally showed a more stable transcript level in the different treatments. However, a similarity between the cytosolic and chloroplastic levels of CuZn-Sod transcripts could be observed in different treatments. This suggests that the redox state of glutathione plays an important role in the in vivo regulation of CuZn-Sod gene expression in plants. Springer-Verlag, 1996 Gene expression nationallicence Glutathione nationallicence Glutathione reductase nationallicence Pinus nationallicence Redox signal transduction nationallicence Superoxide dismutase nationallicence cp : chloroplastic nationallicence cyt : cytosolic nationallicence Gor : gene encoding GR nationallicence GR : glutathione reductase nationallicence GSH : reduced form of glutathione nationallicence GSSG : oxidized form of glutathione nationallicence ROIs : reactive oxygen intermediates nationallicence SOD : superoxide dismutase nationallicence Sod : gene encoding SOD nationallicence Wingsle Gunnar Department of Forest Genetics and Plant Physiology, Faculty of Forestry, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden aut Karpinski Stanislaw Department of Forest Genetics and Plant Physiology, Faculty of Forestry, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden aut Planta Springer-Verlag 198/1(1996-01-01), 151-157 0032-0935 198:1<151 1996 198 425 https://doi.org/10.1007/BF00197598 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197598 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wingsle Gunnar Department of Forest Genetics and Plant Physiology, Faculty of Forestry, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden aut NATIONALLICENCE 700 1- Karpinski Stanislaw Department of Forest Genetics and Plant Physiology, Faculty of Forestry, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/1(1996-01-01), 151-157 0032-0935 198:1<151 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer