caa a22 4500 47710066X CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00197588 doi (NATIONALLICENCE)springer-10.1007/BF00197588 Analysis of late-blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin-like protein [Elektronische Daten] [Baolong Zhu, Tony Chen, Paul Li] The expression patterns of plant defense genes encoding osmotin and osmotin-like proteins imply a dual function in osmotic stress and plant pathogen defense. We have produced transgenic potato (Solanum commersonii Dun.) plants constitutively expressing sense or antisense RNAs from chimeric gene constructs consisting of the cauliflower mosaic virus 35S promoter and a cDNA (pA13) for an osmotin-like protein. Transgenic potato plants expressing high levels of the pA13 osmotin-like protein showed an increased tolerance to the late-blight fungus Phytophthora infestans at various phases of infection, with a greater resistance at an early phase of fungal infection. There was a decrease in the accumulation of osmotin-like mRNAs and proteins when antisense transformants were challenged by fungal infection, although the antisense transformants did not exhibit any alterations in disease susceptibility. Expression of pA13 sense and antisense RNAs had no effect on the development of freezing tolerance in transgenic plants when assayed under a variety of conditions including treatments with abscisic acid or low temperature. These results provide evidence of antifungal activity for a potato osmotin-like protein against the fungus P. infestans, but do not indicate that pA13 osmotin-like protein is a major determinant of freezing tolerance. Springer-Verlag, 1996 Late-blight disease nationallicence Osmotin-like proteins nationallicence Pathogenesis-related proteins nationallicence Phytophthora nationallicence Sense and antisense RNAs nationallicence Solanum nationallicence ABA : abscisic acid nationallicence CaMV : cauliflower mosaic virus nationallicence PR : pathogenesis-related nationallicence T0 : primary transformant nationallicence Zhu Baolong Laboratory of Plant Hardiness, Department of Horticultural Sciences and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, MN, USA aut Chen Tony Department of Horticulture, Center for Gene Research and Biotechnology, Oregon State University, 97331, Corvallis, OR, USA aut Li Paul Laboratory of Plant Hardiness, Department of Horticultural Sciences and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, MN, USA aut Planta Springer-Verlag 198/1(1996-01-01), 70-77 0032-0935 198:1<70 1996 198 425 https://doi.org/10.1007/BF00197588 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197588 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Baolong Laboratory of Plant Hardiness, Department of Horticultural Sciences and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, MN, USA aut NATIONALLICENCE 700 1- Chen Tony Department of Horticulture, Center for Gene Research and Biotechnology, Oregon State University, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Li Paul Laboratory of Plant Hardiness, Department of Horticultural Sciences and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, MN, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/1(1996-01-01), 70-77 0032-0935 198:1<70 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer