caa a22 4500 477100686 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620053 doi (NATIONALLICENCE)springer-10.1007/BF00620053 Chilling, oxidative stress and antioxidant responses in Arabidopsis thaliana callus [Elektronische Daten] [Damian O'Kane, Vera Gill, Patricia Boyd, Roy Burdon] Chilling ofArabidopsis thaliana (L.) Heynh. callus tissue to 4 °C led to conditions of oxidative stress, as indicated by increased levels of the products of peroxidative damage to cell membranes. Cellular H2O2 was also observed to increase initially upon chilling but by day 8 cellular levels had declined to below control levels. Although levels of catalase activity remained similar to those in unchilled tissue, activity of ascorbate peroxidase increased between days 4 and 8 of chilling to 4 °C. In callus held at 23 °C, levels of reduced glutathione remained static whereas they rose in callus held at 4 °C. Levels of oxidised glutathione were initially low but increased significantly by day 4 in the chilled callus. At 23 °C, however, levels of oxidised glutathione remained low. Between days 1 and 3 at 4 °C, levels of glutathione reductase activity increased but by day 8 glutathione reductase activity was similar to that in cells held at 23 °C. Exposure of callus to abscisic acid at 23 °C also led to increased activities of ascorbate peroxidase and glutathione reductase. Springer-Verlag, 1996 Antioxidant enzymes nationallicence Arabidopsis nationallicence Chilling stress nationallicence Glutathione nationallicence Hydrogen peroxide nationallicence Lipid peroxidation nationallicence ABA : abscisic acid nationallicence GSH : reduced glutathione nationallicence GSSG : oxidised glutathione nationallicence TTC : 2′,3′5′-triphenyltetrazolium chloride nationallicence O'Kane Damian Department of Bioscience and Biotechnology, University of Strathclyde, G4 0NR, Glasgow, UK aut Gill Vera Department of Bioscience and Biotechnology, University of Strathclyde, G4 0NR, Glasgow, UK aut Boyd Patricia Department of Bioscience and Biotechnology, University of Strathclyde, G4 0NR, Glasgow, UK aut Burdon Roy Department of Bioscience and Biotechnology, University of Strathclyde, G4 0NR, Glasgow, UK aut Planta Springer-Verlag 198/3(1996-09-01), 371-377 0032-0935 198:3<371 1996 198 425 https://doi.org/10.1007/BF00620053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- O'Kane Damian Department of Bioscience and Biotechnology, University of Strathclyde, G4 0NR, Glasgow, UK aut NATIONALLICENCE 700 1- Gill Vera Department of Bioscience and Biotechnology, University of Strathclyde, G4 0NR, Glasgow, UK aut NATIONALLICENCE 700 1- Boyd Patricia Department of Bioscience and Biotechnology, University of Strathclyde, G4 0NR, Glasgow, UK aut NATIONALLICENCE 700 1- Burdon Roy Department of Bioscience and Biotechnology, University of Strathclyde, G4 0NR, Glasgow, UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 371-377 0032-0935 198:3<371 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer