caa a22 4500 477100694 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620059 doi (NATIONALLICENCE)springer-10.1007/BF00620059 Abscisic acid causes changes in gene expression involved in the induction of the landform of the liverwort Riccia fluitans L [Elektronische Daten] [Elke Hellwege, Karl-Josef Dietz, Wolfram Hartung] The conversion of the submerged form ofRiccia fluitans to the landform either by transfer to a moist solid surface or by treatment with abscisic acid (ABA), is accompanied by the formation of a set of new polypeptides and concomitant down-regulation of other polypeptides. Changes in gene expression were analyzed by twodimensional separations of proteins and differential screening of a cDNA library. One of the landform-specific proteins might depend on the expression of the newly discoveredRic 1 gene. The deduced amino acid sequence of the isolatedRic 1 cDNA clone codes for a protein with a molecular mass of 30.1 kDa. This polypeptide possesses two amino acid sequences which are repeated five times each and it is largely hydrophilic with the exception of a hydrophobic carboxyl-terminal region. Under ABA treatment the expression of theRic 1 mRNA had already reached its maximum after 1 h of incubation. Transferring submerged thalli onto an agar surface resulted in a slower induction. TheRic 1 gene product shows homology to an embryo-specific polypeptide of carrot seeds and to the group 3 of late-embryogenesis-abundant (LEA) proteins. Interestingly, ABA treatment improved the desiccation tolerance of the submerged thalli. Additionally, ABA stimulated the synthesis of a protein which is immunologically related to a tonoplast protein. This finding, together with the fact that the ABA-induced landform exhibits an increased activity of several vacuolar enzymes, may indicate a special role of the tonoplast and the vacuole during ABA-induced conversion of the thallus from the submerged to the terrestrial form. Springer-Verlag, 1996 Abscisic acid nationallicence Drought tolerance nationallicence Protein synthesis nationallicence Riccia nationallicence Vacuole nationallicence ABA : abscisic acid nationallicence 2-D PAGE : two-dimensional polyacrylamide gel electrophoresis nationallicence Fo, Fm : initial and maximal chlorophylla fluorescence nationallicence LEA : late embryogenesis abundant nationallicence Q a : primary quinone acceptor of PS II nationallicence Hellwege Elke Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082, Würzburg, Germany aut Dietz Karl-Josef Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082, Würzburg, Germany aut Hartung Wolfram Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082, Würzburg, Germany aut Planta Springer-Verlag 198/3(1996-09-01), 423-432 0032-0935 198:3<423 1996 198 425 https://doi.org/10.1007/BF00620059 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620059 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hellwege Elke Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082, Würzburg, Germany aut NATIONALLICENCE 700 1- Dietz Karl-Josef Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082, Würzburg, Germany aut NATIONALLICENCE 700 1- Hartung Wolfram Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082, Würzburg, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 423-432 0032-0935 198:3<423 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 477100694 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer