caa a22 4500 477100708 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620051 doi (NATIONALLICENCE)springer-10.1007/BF00620051 An analysis of vacuole development in oat aleurone protoplasts [Elektronische Daten] [T. Emyr Davies, Susan Steele, David Walker, Roger Leigh] Cultured oat (Avena sativa L. — naked form) aleurone protoplasts were employed as a model system for following changes which accompany the development of vacuoles during in-vitro incubation. Over a 5-d period, the aleurone grains progressively grew and fused to form a large central vacuole and the volume of the protoplasts increased sevenfold. The growth of the vacuole was accompanied by a progressive acidification of the vacuolar sap. Vacuolation was inhibited by high concentrations of mannitol and by cycloheximide and cordycepin applied at various times during the incubation period. Neither cycloheximide nor cordycepin affected the initial phases of vacuolation but cycloheximide retarded subsequent stages, particularly if added early in the incubation. Cordycepin inhibited only the later stages of vacuolation. Radiolabelling studies identified at least three novel microsomal proteins, with relative molecular masses of approximately 34, 47 and 48 kDa, which appeared during vacuolation and whose synthesis was markedly affected by these inhibitors. Springer-Verlag, 1996 Avena (aleurone) nationallicence Membrane protein (microsomal) nationallicence Protoplast nationallicence Tonoplast intrinsic Protein Vacuole nationallicence CF : carboxyfluorescein nationallicence CFDA : 6 carboxyfluorescein diacetate nationallicence TIP : tonoplast intrinsic protein nationallicence Emyr Davies T. Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK aut Steele Susan Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK aut Walker David Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK aut Leigh Roger Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK aut Planta Springer-Verlag 198/3(1996-09-01), 356-364 0032-0935 198:3<356 1996 198 425 https://doi.org/10.1007/BF00620051 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620051 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Emyr Davies T. Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK aut NATIONALLICENCE 700 1- Steele Susan Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK aut NATIONALLICENCE 700 1- Walker David Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK aut NATIONALLICENCE 700 1- Leigh Roger Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 356-364 0032-0935 198:3<356 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer