caa a22 4500 477100724 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620049 doi (NATIONALLICENCE)springer-10.1007/BF00620049 Introduction of sense and antisense cDNA for branching enzyme in the amylose-free potato mutant leads to physico-chemical changes in the starch [Elektronische Daten] [E. Flipse, L. Suurs, C. Keetels, J. Kossmann, E. Jacobsen, R. Visser] One isoform of the branching enzyme (BE; EC 2.4.1.18) of potato (Solarium tuberosum L.) is known and catalyses the formation of α-1,6 bonds in a glucan chain, resulting in the branched starch component amylopectin. Constructs containing the antisense or sense-orientated distal 1.5-kb part of a cDNA for potato BE were used to transform the amylose-free (amf) mutant of potato, the starch of which stains red with iodine. The expression of the endogenous BE gene was inhibited either largely or fully as judged by the decrease or absence of the BE mRNA and protein. This resulted in a low percentage of starch granules with a small blue core and large red outer layer. There was no effect on the amylose content, degree of branching or λmax of the iodine-stained starch. However, when the physico-chemical properties of the different starch suspensions were assessed, differences were observed, which although small indicated that starch in the transformants was different from that of theamf mutant. Springer-Verlag, 1996 Antisense inhibition nationallicence Branching enzyme (potato) nationallicence Co-suppression (gene activity) nationallicence Gene activity (branching enzyme) nationallicence Solanum (branching enzyme) nationallicence Starch physico chemical properties nationallicence amf : amylose-free nationallicence BE : branching enzyme nationallicence G′ : storage modulus nationallicence λmax : wavelength with maximal absorption of iodine-stained starch nationallicence Flipse E. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut Suurs L. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut Keetels C. Department of Food Science, Agricultural University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut Kossmann J. Institute für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany aut Jacobsen E. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut Visser R. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut Planta Springer-Verlag 198/3(1996-09-01), 340-347 0032-0935 198:3<340 1996 198 425 https://doi.org/10.1007/BF00620049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Flipse E. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Suurs L. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Keetels C. Department of Food Science, Agricultural University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Kossmann J. Institute für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany aut NATIONALLICENCE 700 1- Jacobsen E. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Visser R. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 340-347 0032-0935 198:3<340 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer