caa a22 4500 477100732 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620063 doi (NATIONALLICENCE)springer-10.1007/BF00620063 Immunochemical comparison of membrane-associated and secreted arabinogalactan-proteins in rice and carrot [Elektronische Daten] [Margaret Smallwood, Edwin Yates, William Willats, Helen Martin, J. Knox] Arabinogalactan-proteins (AGPs) occurring in suspension-cultured rice (Oryza saliva L.) cells, their conditioned medium and at the rice root apex were investigated using monoclonal antibodies and the AGP-binding β-glucosyl Yariv reagent (β GlcY). A monoclonal antibody, LM2, was generated that recognized an acidic carbohydrate epitope common to two soluble AGPs occurring in the conditioned medium of proliferating rice cells, membrane-associated AGPs (rmAGP) in the cultured cells and two AGPs at the rice root apex. In addition, LM2 recognized AGPs secreted by suspensioncultured carrot (Daucus carota L.) cells. The two AGPs of the rice culture medium, srAGP1 and srAGP2, were discriminated by their mobilities during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, reaction with β GlcY, the presence of arabinogalactan epitopes and anion-exchange chromatography. The association of rmAGP with the plasma membrane was investigated by Triton-X-114/aqueous partitioning of both microsomal and plasma-membrane preparations and rmAGP was found to partition into the detergent phase, indicating that AGPs are hydrophobic plasma-membrane proteins in rice. This was in contrast to plasma-membrane AGPs of suspension-cultured carrot cells that partitioned into the aqueous phase. At the rice root apex most of the AGP was associated with the microsomal fraction and also partitioned into the detergent phase, although a distinct highmolecular-mass AGP entered the aqueous phase. Springer-Verlag, 1996 Arabinogalactan-protein nationallicence Cell surface (plant) nationallicence Daucus nationallicence Monoclonal antibody nationallicence Oryza nationallicence Plasma membrane (plant) nationallicence AGP : arabinogalactan-protein nationallicence βGlcY : β-glucosyl Yariv reagent nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence Smallwood Margaret Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut Yates Edwin Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut Willats William Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut Martin Helen Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut Knox J. Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut Planta Springer-Verlag 198/3(1996-09-01), 452-459 0032-0935 198:3<452 1996 198 425 https://doi.org/10.1007/BF00620063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smallwood Margaret Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Yates Edwin Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Willats William Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Martin Helen Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Knox J. Centre for Plant Biochemistry & Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 452-459 0032-0935 198:3<452 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer