caa a22 4500 477100813 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620060 doi (NATIONALLICENCE)springer-10.1007/BF00620060 Induction of embryogenesis with colchicine instead of heat in microspores of Brassica napus L. cv. Topas [Elektronische Daten] [Ji-Ping Zhao, Daina Simmonds, William Newcomb] Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis fromBrassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction inB. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development. Springer-Verlag, 1996 Brassica napus nationallicence Colchicine nationallicence Heat shock nationallicence Microspore embryogenesis nationallicence Microtubules nationallicence LU : Late-unicellular nationallicence PPB : Preprophase band nationallicence UV : unicellular-vacuolate nationallicence Zhao Ji-Ping Department of Biology, Queen's University, K7L 3N6, Kingston, ON, Canada aut Simmonds Daina Plant Research Centre, Agriculture Canada, CEF#21, K1A 0C6, Ottawa, ON, Canada aut Newcomb William Department of Biology, Queen's University, K7L 3N6, Kingston, ON, Canada aut Planta Springer-Verlag 198/3(1996-09-01), 433-439 0032-0935 198:3<433 1996 198 425 https://doi.org/10.1007/BF00620060 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620060 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Ji-Ping Department of Biology, Queen's University, K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 700 1- Simmonds Daina Plant Research Centre, Agriculture Canada, CEF#21, K1A 0C6, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Newcomb William Department of Biology, Queen's University, K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 433-439 0032-0935 198:3<433 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer