caa a22 4500 477100821 CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620067 doi (NATIONALLICENCE)springer-10.1007/BF00620067 Phytochrome- and blue-light-absorbing pigment-mediated directional movement of chloroplasts in dark-adapted prothallial cells of fern Adiantum as analyzed by microbeam irradiation [Elektronische Daten] [Takatoshi Kagawa, Masarnitsu Wada] When prothalli ofAdiantum capillus-veneris L. were kept for 2 d in the dark, chloroplasts gathered along the anticlinal walls (Kagawa and Wada, 1994, J Plant Res 107: 389-398). In these dark-adapted prothallial cells, irradiation with a microbeam (10 gm in diameter) of red (R) or blue light (B) for 60 s moved the chloroplasts towards the irradiated locus during a subsequent dark period. Chloroplasts located less than 20 gm from the center of the R microbeam (18 J·m−2) moved towards the irradiated locus. The higher the fluence of the light, the greater the distance from which chloroplasts could be attracted. The B microbeam was less effective than the R microbeam. Chloroplasts started to move anytime up to 20 min after the R stimulus, but with the B microbeam the effect of the stimulus was usually apparent within 10 min after irradiation. The velocity of chloroplast migration was independent of light-fluence in both R and B and was about - 0.3 μm·min−1 between 15 min and 30 min after irradiation. Whole-cell irradiation with far-red light immediately after R- and B-microbeam irradiations demonstrated that these responses were mediated by phytochrome and a blue-light-absorbing pigment, respectively. Sequential treatment with R and B microbeams, whose fluence rates were less than the threshold values when applied separately, resulted in an additive effect and induced chloroplast movement, strongly suggesting that signals from phytochrome and the blue-light-absorbing pigment could interact at some point before the induction of chloroplast movement. Springer-Verlag, 1996 Adiantum (prothallial cell) nationallicence Blue-lightabsorbing pigment nationallicence Chloroplast movement (directional) nationallicence Microbeam irradiation nationallicence Phytochrome nationallicence Signal-transduction chain nationallicence B : blue light nationallicence FR : far-red light nationallicence IR : infrared light nationallicence R : red light nationallicence Kagawa Takatoshi Laboratory of Photoperception and Signal Transduction, Frontier Research System, RIKEN Institute, Hirosawa 2-1, 351-01, Wako, Saitama, Japan aut Wada Masarnitsu Department of Biology, Faculty of Science, Tokyo Metropolitan University, Minami Ohsawa 1-1, 192-03, Hachicji, Tokyo, Japan aut Planta Springer-Verlag 198/3(1996-09-01), 488-493 0032-0935 198:3<488 1996 198 425 https://doi.org/10.1007/BF00620067 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620067 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kagawa Takatoshi Laboratory of Photoperception and Signal Transduction, Frontier Research System, RIKEN Institute, Hirosawa 2-1, 351-01, Wako, Saitama, Japan aut NATIONALLICENCE 700 1- Wada Masarnitsu Department of Biology, Faculty of Science, Tokyo Metropolitan University, Minami Ohsawa 1-1, 192-03, Hachicji, Tokyo, Japan aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 488-493 0032-0935 198:3<488 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer