caa a22 4500 47710083X CHVBK 20180405111540.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620065 doi (NATIONALLICENCE)springer-10.1007/BF00620065 Characterization of a novel drought-induced 34-kDa protein located in the thylakoids of Solanum tuberosum L. plants [Elektronische Daten] [Ghislaine Pruvot, Stéphan Cuiné, Gilles Peltier, Pascal Rey] Using two-dimensional electrophoresis and Coomassie Blue staining, the accumulation of a 34-kDa protein (named cdsp 34 for chloroplastic drought-induced stress protein) is shown in the thylakoids ofSolanum tuberosum plants subjected to a progressive and reversible water deficit. In-vivo labeling experiments showed an increased synthesis of cdsp 34 from the early stages of drought stress (leaf relative water content around 85%) and throughout the constraint. Sequences of the N-terminal part and of four tryptic-digest peptides did not reveal significant homology between the cdsp 34 protein and other known proteins. Western blotting analysis, using a serum raised against the N-terminal part of cdsp 34, confirmed the accumulation of cdsp 34 in thylakoids upon drought stress. From immunoblot analysis of different chloroplastic subfractions, the cdsp 34 protein appears to be an extrinsic protein preferentially located in unstacked stroma thylakoids. Immunoprecipitation of in-vitro-translated products, as well as Southern analysis, showed that the cdsp 34 protein is nuclear encoded. After rewatering of water-stressed plants, the level of cdsp 34 synthesis was reduced, but remained substantially higher than in control plants. Western analysis showed the persistence of a high amount of cdsp 34 in rewatered plants for at least two weeks. Based on the abundance and on the location of cdsp 34 within thylakoids, a putative role for this novel chloroplastic protein is discussed in relation to the tolerance of the photosynthetic apparatus of higher plants to dehydration. Springer-Verlag, 1996 Chloroplast nationallicence Drought-induced protein nationallicence Solanum nationallicence Thylakoid protein nationallicence Water deficit nationallicence 2-D : two-dimensional nationallicence CF : coupling factor nationallicence cdsp : chloroplastic drought-induced stress protein nationallicence dsp : desiccation stress protein nationallicence LHC : light-harvesting complex nationallicence PVDF : polyvinylidene fluoride nationallicence RWC : relative water content nationallicence Pruvot Ghislaine Département d'Ecophysiologie Végétale et de Microbiologie Centre d'Etudes de Cadarache, CEA, Laboratoire d'Ecophysiologie de la Photosynthèse, F-13108, Saint-Paul-lez-Durance, France aut Cuiné Stéphan Département d'Ecophysiologie Végétale et de Microbiologie Centre d'Etudes de Cadarache, CEA, Laboratoire d'Ecophysiologie de la Photosynthèse, F-13108, Saint-Paul-lez-Durance, France aut Peltier Gilles Département d'Ecophysiologie Végétale et de Microbiologie Centre d'Etudes de Cadarache, CEA, Laboratoire d'Ecophysiologie de la Photosynthèse, F-13108, Saint-Paul-lez-Durance, France aut Rey Pascal Département d'Ecophysiologie Végétale et de Microbiologie Centre d'Etudes de Cadarache, CEA, Laboratoire d'Ecophysiologie de la Photosynthèse, F-13108, Saint-Paul-lez-Durance, France aut Planta Springer-Verlag 198/3(1996-09-01), 471-479 0032-0935 198:3<471 1996 198 425 https://doi.org/10.1007/BF00620065 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620065 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pruvot Ghislaine Département d'Ecophysiologie Végétale et de Microbiologie Centre d'Etudes de Cadarache, CEA, Laboratoire d'Ecophysiologie de la Photosynthèse, F-13108, Saint-Paul-lez-Durance, France aut NATIONALLICENCE 700 1- Cuiné Stéphan Département d'Ecophysiologie Végétale et de Microbiologie Centre d'Etudes de Cadarache, CEA, Laboratoire d'Ecophysiologie de la Photosynthèse, F-13108, Saint-Paul-lez-Durance, France aut NATIONALLICENCE 700 1- Peltier Gilles Département d'Ecophysiologie Végétale et de Microbiologie Centre d'Etudes de Cadarache, CEA, Laboratoire d'Ecophysiologie de la Photosynthèse, F-13108, Saint-Paul-lez-Durance, France aut NATIONALLICENCE 700 1- Rey Pascal Département d'Ecophysiologie Végétale et de Microbiologie Centre d'Etudes de Cadarache, CEA, Laboratoire d'Ecophysiologie de la Photosynthèse, F-13108, Saint-Paul-lez-Durance, France aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 471-479 0032-0935 198:3<471 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer