caa a22 4500 477100848 CHVBK 20180405111541.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00620055 doi (NATIONALLICENCE)springer-10.1007/BF00620055 Isolation of high-chlorophyll-fluorescence mutants of Arabidopsis thaliana and their characterisation by spectroscopy, immunoblotting and Northern hybridisation [Elektronische Daten] [Jörg Meurer, Karin Meierhoff, Peter Westhoff] Thirty-four recessive photosynthetic mutants of the high-chlorophyll-fluorescence (hcf) phenotype have been isolated by screening 7700 M2 progenies of ethyl methane sulfonate-treated seeds ofArabidopsis thaliana. Most of the mutants isolated were found to be seedlinglethal, but could be grown on sucrose-supplemented media. Chlorophyll (Chl) fluorescence induction, absorption changes in the reaction-centre chlorophyll of PS I (P700) at 830 nm and Chla/Chlb ratios were recorded in order to probe the photosynthetic functions and to define the mutational lesion. These studies were complemented by immunoblot and Northern analyses which finally led to the classification of the mutants into six different groups. Four classes of mutants were affected in PS I, PS II (two different classes) or the intersystem electron-transport chain, respectively. A fifth mutant class was of pleiotropic nature and the sixth class comprised a Chlb-deficient mutant. Several of the mutants showed severe deficiencies in the levels of subunits of PS I, PS II or the cytochromeb 6/f complex. Thus the mutational lesions could be located precisely. Only one mutant was defective in the transcript patterns of some plastid-encoded photosynthesis genes. Hence most of the mutants isolated appear to be affected in translational and post-translational regulatory processes of thylakoid membrane biogenesis or in structural genes encoding constituent subunits of the thylakoid protein complexes. Springer-Verlag, 1996 Arabidopsis nationallicence High-chlorophyll-fluorescence mutants nationallicence Photosynthesis nationallicence Chl : chlorophyll nationallicence ΔA : in-vivo absorbance change of P700 at 830 nm measured immediately after interruption of the actinic white light which had been given for four minutes nationallicence ΔAmax : in-vivo absorbance change of P700 at 830 nm induced by background far-red light at 720 nm nationallicence EMS : ethyl methane sulfonate nationallicence Fo and Fm : minimal and maximal Chla fluorescence of dark-adapted leaves nationallicence F′o, F′m and Fs : minimal, maximal and steady-state Chla fluorescence of light-adapted leaves nationallicence Fv : variable fluorescence nationallicence ΦI : quantum yield of PS I nationallicence ΦII : quantum yield of PS II nationallicence hcf : high chlorophyll fluorescence nationallicence P700 : reaction-centre chlorophyll of PS I nationallicence qN : non-photochemical quenching nationallicence qp : photochemical quenching nationallicence Meurer Jörg Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut Meierhoff Karin Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut Westhoff Peter Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut Planta Springer-Verlag 198/3(1996-09-01), 385-396 0032-0935 198:3<385 1996 198 425 https://doi.org/10.1007/BF00620055 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00620055 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meurer Jörg Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Meierhoff Karin Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Westhoff Peter Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/3(1996-09-01), 385-396 0032-0935 198:3<385 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer