caa a22 4500 477100902 CHVBK 20180405111541.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00262639 doi (NATIONALLICENCE)springer-10.1007/BF00262639 Comparison of mechanisms controlling uptake and accumulation of 2,4-dichlorophenoxy acetic acid, naphthalene-1-acetic acid, and indole-3-acetic acid in suspension-cultured tobacco cells [Elektronische Daten] [Akin Delbarre, Philippe Muller, Viviane Imhoff, Jean Guern] Accumulation of radiolabelled naphthalene-1-acetic acid (1-NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and indole-3-acetic acid (IAA) has been measured in suspension-cultured tobacco (Nicotiana tabacum) cells. In this paper is presented a simple methodology allowing activities of the auxin influx and efflux carriers to be monitored independently by measuring the cellular accumulation of [3H]NAA and [14C]2,4-D. We have shown that 1-NAA enters cells by passive diffusion and has its accumulation level controlled by the efflux carrier. By contrast, 2,4-D uptake is mostly ensured by the influx carrier and this auxin is not secreted by the efflux carrier. Both auxin carriers contribute to IAA accumulation. The kinetic parameters and specificity of each carrier have been determined and new information concerning interactions with naphthylphthalamic acid, pyrenoylbenzoic acid, and naphthalene-2-acetic acid are provided. The relative contributions of diffusion and carrier-mediated influx and efflux to the membrane transport of 2,4-D, 1-NAA, and IAA have been quantified, and the data indicate that plant cells are able to modulate over a large range their auxin content by modifying the activity of each carrier. Springer-Verlag, 1996 Auxin carriers nationallicence Auxin membrane transport nationallicence Nicotiana (suspension-cultured cells) nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence 1-NAA : naphthalene-1-acetic acid nationallicence 2-NAA : naphthalene-2-acetic acid nationallicence NPA : N-1-naphthylphthalamic acid nationallicence PBA : 2-(1-pyrenoyl)benzoic acid nationallicence Vm : maximum transport capacity of the carrier nationallicence Delbarre Akin Institut des Sciences Végétales, CNRS, Batiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France aut Muller Philippe Institut des Sciences Végétales, CNRS, Batiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France aut Imhoff Viviane Institut des Sciences Végétales, CNRS, Batiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France aut Guern Jean Institut des Sciences Végétales, CNRS, Batiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France aut Planta Springer-Verlag 198/4(1996-04-01), 532-541 0032-0935 198:4<532 1996 198 425 https://doi.org/10.1007/BF00262639 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00262639 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Delbarre Akin Institut des Sciences Végétales, CNRS, Batiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France aut NATIONALLICENCE 700 1- Muller Philippe Institut des Sciences Végétales, CNRS, Batiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France aut NATIONALLICENCE 700 1- Imhoff Viviane Institut des Sciences Végétales, CNRS, Batiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France aut NATIONALLICENCE 700 1- Guern Jean Institut des Sciences Végétales, CNRS, Batiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/4(1996-04-01), 532-541 0032-0935 198:4<532 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer