caa a22 4500 477100953 CHVBK 20180405111541.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00262650 doi (NATIONALLICENCE)springer-10.1007/BF00262650 Cyclopiazonic acid disturbs the regulation of cytosolic calcium when repetitive action potentials are evoked in Dionaea traps [Elektronische Daten] [Kazimierz Trebacz, Marion Busch, Zygmunt Hejnowicz, Andreas Sievers] Evoking of action potentials (APs) in the trap of Dionaea muscipula Ellis at intervals shorter than 20 s caused a gradual decrease in the amplitude of the APs. At longer intervals the amplitude was constant. The calcium ionophore A23187 (1 μM) caused a considerable decrease of AP amplitude. Pretreatment of a segment of the Dionaea trap with cyclopiazonic acid (CPA), which is a specific inhibitor of the Ca2+-ATPase in the sarcoplasmic seticulum of animal cells and in ER vesicles isolated from plant cells, only slightly affected the amplitude when APs were evoked every 10 min; however, it caused a considerable decrease in the amplitude when the stimulation was repeated every 2 min. Assuming that APs increase the concentration of cytosolic Ca2+ and the amplitude of AP depends on the gradient of Ca2+ across the plasma membrane, the effect of CPA on the AP amplitude indicates that CPA inhibits the sequestration of Ca2+ in Dionaea cells. Springer-Verlag, 1996 Action potential nationallicence Cyclopiazonic acid nationallicence Cytosolic calcium nationallicence Dionaea (action potential, calcium) nationallicence AP : action potential nationallicence [Ca2+]c : free Ca2+ concentration in the cytosol nationallicence CPA : cyclopiazonic acid nationallicence DMSO : dimethylsulfoxide nationallicence PM : plasma membrane nationallicence Trebacz Kazimierz Botanisches Institut, Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut Busch Marion Botanisches Institut, Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut Hejnowicz Zygmunt Botanisches Institut, Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut Sievers Andreas Botanisches Institut, Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut Planta Springer-Verlag 198/4(1996-04-01), 623-626 0032-0935 198:4<623 1996 198 425 https://doi.org/10.1007/BF00262650 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00262650 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Trebacz Kazimierz Botanisches Institut, Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut NATIONALLICENCE 700 1- Busch Marion Botanisches Institut, Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut NATIONALLICENCE 700 1- Hejnowicz Zygmunt Botanisches Institut, Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut NATIONALLICENCE 700 1- Sievers Andreas Botanisches Institut, Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/4(1996-04-01), 623-626 0032-0935 198:4<623 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer