caa a22 4500 477100961 CHVBK 20180405111541.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00262652 doi (NATIONALLICENCE)springer-10.1007/BF00262652 Variations in the structure of neutral sugar chains in the pectic polysaccharides of morphologically different carrot calli and correlations with the size of cell clusters [Elektronische Daten] [A. Kikuchi, Y. Edashige, T. Ishii, T. Fujii, S. Satoh] Carrot (Daucus carota L.) embryogenic callus (EC) loses its embryogenic competence and becomes nonembryogenic callus (NC) during long-term culture. With the loss of embryogenic competence, the cell clusters become smaller and the extent of intercellular attachments is reduced. Pectic fractions prepared from EC and NC were separated into two subfractions by gel filtration. A difference in sugar composition between EC and NC was found only in the high-molecular-mass (ca. 1300 kDa) subfraction, and the ratio of the amount of arabinose to that of galactose (Ara/Gal) was strongly and positively correlated with the size of cell clusters in several different cultures. From the results of sugar-composition and methylation analyses, and the results of treatment with exo-arabinanase, models of the neutral sugar chains of pectins from EC and NC are proposed. Both neutral sugar chains are composed of three regions. The basal region is composed of linearly linked arabinan 5-Araf> moieties in both types of callus. The middle galactan region is composed of 6-linked galactose, some of which branches at the 3 and 4 positions, and this region is larger and more frequently branched in NC than in EC. Finally, the terminal arabinan region is composed of 5-linked arabinose, branched at the 3 position, and the size of the terminal arabinan is larger in EC than in NC. The significance of the neutral sugar chains of pectins in the interaction of cell wall components and intercellular attachment is discussed. Springer-Verlag, 1996 Callus nationallicence Cell wall nationallicence Daucus (cell walls) nationallicence Morphogenesis nationallicence Neutral sugar nationallicence Pectin nationallicence Ara/Gal : ratio (w/w) of the amount of arabinose to that of galactose nationallicence EC : embryogenic callus nationallicence NC : non-embryogenic callus nationallicence T-Araf : terminal arabinose nationallicence Kikuchi A. Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut Edashige Y. Forestry and Forest Products Research Institute Tsukuba, 305, Ibaraki, Japan aut Ishii T. Forestry and Forest Products Research Institute Tsukuba, 305, Ibaraki, Japan aut Fujii T. Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut Satoh S. Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut Planta Springer-Verlag 198/4(1996-04-01), 634-639 0032-0935 198:4<634 1996 198 425 https://doi.org/10.1007/BF00262652 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00262652 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kikuchi A. Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Edashige Y. Forestry and Forest Products Research Institute Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ishii T. Forestry and Forest Products Research Institute Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Fujii T. Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Satoh S. Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/4(1996-04-01), 634-639 0032-0935 198:4<634 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer