caa a22 4500 477101011 CHVBK 20180405111541.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00262638 doi (NATIONALLICENCE)springer-10.1007/BF00262638 Changes in intracellular amino acids and organic acids induced by nitrogen starvation and nitrate or ammonium resupply in the cyanobacterium Phormidium laminosum [Elektronische Daten] [María Tapia, Jesús de Alda, María Llama, Juan Serra] In Phormidium laminosum cells, nitrogen starvation caused a decrease in the intracellular levels of all amino acids, except glutamate, and an increase in the total level of the analyzed organic acids. The addition of nitrate or ammonium to N-starved cells resulted in substantial increases in the pool size of most amino acids. Upon addition of ammonium the total level of organic acids diminished, whereas it increased upon addition of nitrate, after a transient decay during the first minutes. Nitrogen resupply stimulated amino acid synthesis, the effect being faster and higher when ammonium was assimilated. The data indicate that nitrate and ammonium assimilation induced an enhancement of carbon flow through the glycolytic and the tricarboxylic-acid pathways to amino acid biosynthesis, with a concurrent decrease in the carbohydrate reserves. The results suggest that the availability of carbon skeletons limited the rate of ammonium assimilation, whereas the availability of reducing equivalents limited the rate of nitrate assimilation. Springer-Verlag, 1996 Ammonium assimilation nationallicence Carbon-nitrogen interactions nationallicence Cyanobacteria nationallicence Nitrate assimilation nationallicence Nitrogen starvation nationallicence Phormidium nationallicence Chl : chlorophyll nationallicence GOGAT : ferredoxin-dependent glutamate synthase (EC 1.4.7.1) nationallicence GS : glutamine synthetase (EC 6.3.1.2) nationallicence Tapia María Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, E-48080, Bilbao, Spain aut de Alda Jesús Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, E-48080, Bilbao, Spain aut Llama María Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, E-48080, Bilbao, Spain aut Serra Juan Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, E-48080, Bilbao, Spain aut Planta Springer-Verlag 198/4(1996-04-01), 526-531 0032-0935 198:4<526 1996 198 425 https://doi.org/10.1007/BF00262638 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00262638 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tapia María Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, E-48080, Bilbao, Spain aut NATIONALLICENCE 700 1- de Alda Jesús Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, E-48080, Bilbao, Spain aut NATIONALLICENCE 700 1- Llama María Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, E-48080, Bilbao, Spain aut NATIONALLICENCE 700 1- Serra Juan Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, E-48080, Bilbao, Spain aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/4(1996-04-01), 526-531 0032-0935 198:4<526 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer