caa a22 4500 477101038 CHVBK 20180405111541.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00262646 doi (NATIONALLICENCE)springer-10.1007/BF00262646 Modification of reproductive development in Arabidopsis thaliana under spaceflight conditions [Elektronische Daten] [Anxiu Kuang, Mary Musgrave, Sharon Matthews] Reproductive development in Arabidopsis thaliana (L.) Heynh. cv. Columbia plants was investigated under spaceflight conditions on shuttle mission STS-51. Plants launched just prior to initiation of the reproductive phase developed flowers and siliques during the 10-d flight. Approximately 500 flowers were produced in total by the 12 plants in both the ground control and spaceflight material, and there was no significant difference in the number of flowers in each size class. The flower buds and siliques of the spaceflight plants were not morphologically different from the ground controls. Pollen viability tests immediately post-flight using fluorescein diacetate indicated that about 35% of the pollen was viable in the spaceflight material. Light-microscopy observations on this material showed that the female gametophytes also had developed normally to maturity. However, siliques from the spaceflight plants contained empty, shrunken ovules, and no evidence of pollen transfer to stigmatic papillae was found by light microscopy immediately post-flight or by scanning electron microscopy on fixed material. Short stamen length and indehiscent anthers were observed in the spaceflight material, and a film-like substance inside the anther that connected to the tapetum appeared to restrict the release of pollen from the anthers. These observations indicate that given appropriate growing conditions, early reproductive development in A. thaliana can occur normally under spaceflight conditions. On STS-51, reproductive development aborted due to obstacles in pollination or fertilization. Springer-Verlag, 1996 Arabidopsis nationallicence Female gametophyte nationallicence Pollen nationallicence Reproduction nationallicence Spaceflight nationallicence Kuang Anxiu Department of Plant Pathology and Crop Physiology, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, 70803, Baton Rouge, LA, USA aut Musgrave Mary Department of Plant Pathology and Crop Physiology, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, 70803, Baton Rouge, LA, USA aut Matthews Sharon Department of Plant Biology, Louisiana State University, 70803, Baton Rouge, LA, USA aut Planta Springer-Verlag 198/4(1996-04-01), 588-594 0032-0935 198:4<588 1996 198 425 https://doi.org/10.1007/BF00262646 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00262646 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuang Anxiu Department of Plant Pathology and Crop Physiology, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Musgrave Mary Department of Plant Pathology and Crop Physiology, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Matthews Sharon Department of Plant Biology, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/4(1996-04-01), 588-594 0032-0935 198:4<588 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer