caa a22 4500 477101054 CHVBK 20180405111541.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00262641 doi (NATIONALLICENCE)springer-10.1007/BF00262641 Increased endogenous cytokinin in the Arabidopsis amp1 mutant corresponds with de-etiolation responses [Elektronische Daten] [Amy Chin-Atkins, Stuart Craig, Charles Hocart, Elizabeth Dennis, Abdul Chaudhury] The high-cytokinin Arabidopsis thaliana (L.) Heynh. mutant amp1 of has been further characterised. We extend our previous work on the cytokinin level in the amp1 mutant and show that it contains high levels of endogenous cytokinins in both light- and dark-grown plants, and that amp1 can be phenocopied in the dark by growing wild-type plants on exogenous cytokinin. Previously we showed that dark-grown amp1 plants display de-etiolated characteristics such as short unhooked hypocotyls, opened cotyledons and formation of leaves. We further show that amp1 mutants display de-etiolated plastid morphology and increased levels of transcripts of light-regulated genes, indicating that in this mutant light-mediated processes are partially induced in the dark. The amp1 mutant also shows a reduced level of expression of several light-regulated genes compared with the wild type when grown in light, and has an altered dark-adaptation response when compared with the wild type. These results demonstrate an association between high cytokinin levels and de-etiolation, and we infer that cytokinin itself or a cytokinin-mediated process is involved in regulation of etiolation. The map location, phenotypes and de-etiolation responses in the amp1 mutant are different from those of previously described de-etiolated mutants such as det1, det2, cop1 and cop9. We propose a model in which cytokinin acts as a component of the induction of photomorphogenic processes via a signal transduction pathway which is independent of light. Springer-Verlag, 1996 Arabidopsis (mutant) nationallicence Cytokinin nationallicence De-etiolation nationallicence Mutant ( amp1 ) nationallicence Photomorphogenesis nationallicence cab : gene for light-harvesting chlorophyll-a/b binding proteins nationallicence chs : gene for chalocome synthase nationallicence rbcs : gene for small subunit of ribulose-1,5-bisphosphate carboxylase/ oxygenase nationallicence Chin-Atkins Amy CSIRO Division of Plant Industry, G.P.O. Box 1600, 2601, Canberra, ACT, Australia aut Craig Stuart CSIRO Division of Plant Industry, G.P.O. Box 1600, 2601, Canberra, ACT, Australia aut Hocart Charles Cooperative Research Centre for Plant Science, Australian National University, G.P.O. Box 475, 2601, Canberra, ACT, Australia aut Dennis Elizabeth CSIRO Division of Plant Industry, G.P.O. Box 1600, 2601, Canberra, ACT, Australia aut Chaudhury Abdul CSIRO Division of Plant Industry, G.P.O. Box 1600, 2601, Canberra, ACT, Australia aut Planta Springer-Verlag 198/4(1996-04-01), 549-556 0032-0935 198:4<549 1996 198 425 https://doi.org/10.1007/BF00262641 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00262641 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chin-Atkins Amy CSIRO Division of Plant Industry, G.P.O. Box 1600, 2601, Canberra, ACT, Australia aut NATIONALLICENCE 700 1- Craig Stuart CSIRO Division of Plant Industry, G.P.O. Box 1600, 2601, Canberra, ACT, Australia aut NATIONALLICENCE 700 1- Hocart Charles Cooperative Research Centre for Plant Science, Australian National University, G.P.O. Box 475, 2601, Canberra, ACT, Australia aut NATIONALLICENCE 700 1- Dennis Elizabeth CSIRO Division of Plant Industry, G.P.O. Box 1600, 2601, Canberra, ACT, Australia aut NATIONALLICENCE 700 1- Chaudhury Abdul CSIRO Division of Plant Industry, G.P.O. Box 1600, 2601, Canberra, ACT, Australia aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/4(1996-04-01), 549-556 0032-0935 198:4<549 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer