caa a22 4500 477101216 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00206248 doi (NATIONALLICENCE)springer-10.1007/BF00206248 The effect of waxy mutations on the granule-bound starch synthases of barley and maize endosperms [Elektronische Daten] [Christopher Hylton, Kay Denyer, Peter Keeling, Ming-Tang Chang, Alison Smith] The effects of waxy mutations on starch-granule-bound starch synthases (EC 2.4.1.18) in the developing endosperm of barley (Hordeum vulgare L.) and maize (Zea mays L.) have been investigated. Three granule-bound starch synthases in barley endosperm were identified by use of antibodies to known starch synthases, by reconstitution and assay of individual proteins from sodium dodecyl sulphate-polyacrylamide gels of granule-bound proteins, and by partial purification of proteins released by enzymic digestion of starch. These are proteins of 60, 77 and 90 kDa. Use of antibodies to known starch synthases and partial purification of proteins released by enzymic digestion of starch indicated that there may be at least four granule-bound starch synthases in maize endosperm: proteins of 59, 74, 77 and 83 kDa. Mutations at the waxy loci of both species affected only the 60- (barley) and 59-(maize) kDa isoforms. No evidence was found that other putative isoforms are altered in abundance or activity by the mutations. The contribution of our results to understanding of the starch synthase activity of intact starch granules and the mechanism of amylose synthesis is discussed. Springer-Verlag, 1996 Endosperm nationallicence Hordeum nationallicence Starch granule nationallicence Starch synthase nationallicence waxy mutation nationallicence Zea nationallicence Hylton Christopher John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK aut Denyer Kay John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK aut Keeling Peter ExSeed Genetics, 1573 Food Sciences Building, Iowa State University, 50011-1061, Ames, Iowa, USA aut Chang Ming-Tang ExSeed Genetics, 1573 Food Sciences Building, Iowa State University, 50011-1061, Ames, Iowa, USA aut Smith Alison John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK aut Planta Springer-Verlag 198/2(1996-02-01), 230-237 0032-0935 198:2<230 1996 198 425 https://doi.org/10.1007/BF00206248 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00206248 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hylton Christopher John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK aut NATIONALLICENCE 700 1- Denyer Kay John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK aut NATIONALLICENCE 700 1- Keeling Peter ExSeed Genetics, 1573 Food Sciences Building, Iowa State University, 50011-1061, Ames, Iowa, USA aut NATIONALLICENCE 700 1- Chang Ming-Tang ExSeed Genetics, 1573 Food Sciences Building, Iowa State University, 50011-1061, Ames, Iowa, USA aut NATIONALLICENCE 700 1- Smith Alison John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/2(1996-02-01), 230-237 0032-0935 198:2<230 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer