caa a22 4500 477101224 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00206258 doi (NATIONALLICENCE)springer-10.1007/BF00206258 Gravitropism of the primary root of maize: a complex pattern of differential cellular growth in the cortex independent of the microtubular cytoskeleton [Elektronische Daten] [František Baluška, Martin Hauskrecht, Peter Barlow, Andreas Sievers] The spatio-temporal sequence of cellular growth within the post-mitotic inner and outer cortical tissue of the apex of the primary root of maize (Zea mays L.) was investigated during its orthogravitropic response. In the early phase (0-30 min) of the graviresponse there was a strong inhibition of cell lengthening in the outer cortex at the lower side of the root, whereas lengthening was only slightly impaired in the outer cortex at the upper side. Initially, inhibition of differential cell lengthening was less pronounced in the inner cortex indicating that tissue tensions which, in these circumstances, inevitably develop at the outer-inner cortex interface, might help to drive the onset of the root bending. At later stages of the graviresponse (60 min), when a root curvature had already developed, cells of the inner cortex then exhibited a prominent cell length differential between upper and lower sides, whereas the outer cortex cells had re-established similar lengths. Again, tissue tensions associated with the different patterns of cellular behaviour in the inner and outer cortex tissues, could be of relevance in terminating the root bending. The perception of gravity and the complex tissue-specific growth responses both proceeded normally in roots which were rendered devoid of microtubules by colchicine and oryzalin treatments. The lack of involvement of microtubules in the graviresponse was supported by several other lines of evidence. For instance, although taxol stabilized the cortical microtubules and prevented their re-orientation in post-mitotic cortical cells located at the lower side of gravistimulated roots, root bending developed normally. In contrast, when gravistimulated roots were physically prevented from bending, re-oriented arrays of cortical microtubules were seen in all post-mitotic cortical cells, irrespective of their position within the root. Springer-Verlag, 1996 Cell elongation nationallicence Cell shape nationallicence Gravitropism nationallicence Microtubule nationallicence Root growth nationallicence Zea nationallicence CMTs : cortical microtubules nationallicence CW : Cholodny-Went nationallicence FF : form factor nationallicence MT : microtubule nationallicence Baluška František Botanisches Institut der Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut Hauskrecht Martin Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-84223, Bratislava, Slovakia aut Barlow Peter Department of Agricultural Sciences, University of Bristol, IACR — Long Ashton Research Station, Long Ashton, BS18 9AF, Bristol, UK aut Sievers Andreas Botanisches Institut der Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut Planta Springer-Verlag 198/2(1996-02-01), 310-318 0032-0935 198:2<310 1996 198 425 https://doi.org/10.1007/BF00206258 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00206258 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baluška František Botanisches Institut der Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut NATIONALLICENCE 700 1- Hauskrecht Martin Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-84223, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Barlow Peter Department of Agricultural Sciences, University of Bristol, IACR — Long Ashton Research Station, Long Ashton, BS18 9AF, Bristol, UK aut NATIONALLICENCE 700 1- Sievers Andreas Botanisches Institut der Universität Bonn, Venusbergweg 22, D-53115, Bonn, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 198/2(1996-02-01), 310-318 0032-0935 198:2<310 1996 198 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer