caa a22 4500 477101283 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196891 doi (NATIONALLICENCE)springer-10.1007/BF00196891 Molecular analysis of tri -mutant alleles in tomato indicates the Tri locus is the gene encoding the apoprotein of phytochrome B1 [Elektronische Daten] [L. Kerckhoffs, A. Van Tuinen, B. Hauser, M.-M. Cordonnier-Pratt, A. Nagatani, M. Koornneef, L. Pratt, R. Kendrick] Four monogenic recessive tomato (Lycopersicon esculentum Mill.) mutants at the temporarily red light-Insensitive (tri) locus (tri 1, tri 2in the genetic background breeding line GT; tri 3, tri 4in the genetic background cultivar Moneymaker) were studied. These mutants had slightly longer hypocotyls under white light than the wild type (WT). Western-blot analysis showed that the tri 1mutant was deficient in a relatively lightstable phytochrome apoprotein (116 kDa) that was recognized in the WT by an antibody to tobacco phytochrome B; tri 2had a 166-kDa band reduced in abundance; and tri 2and tri 4had bands reduced in molecular mass, approx. 105 and 95 kDa, respectively. These patterns were also found in light-grown plants. Northern-blot analysis for PHYB1 mRNA showed for tri 2a transcript approx. 2 kb larger, for tri 4, a transcript of WT size, but much reduced in abundance and for tri 1and tri 3transcripts equivalent in size and abundance to WT. In these mutants the transcripts of other members of the tomato phytochrome gene family (PHYA, PHYB2, PHYE, PHYF) were indistinguishable in size and abundance from WT. Thus, it appears that the tri locus specifically affects PHYB1 gene expression. Unlike phytochrome-B mutants in other plants, de-etiolated seedlings of the tri mutants exhibited normal responses to end-of-day far-red (EODFR) light and supplementary far-red light during the day. Since the holophytochromes of types B1 and B2 (phyB1 and phyB2) are closely related, it is proposed that there might be redundancy between them for these responses. Springer-Verlag, 1996 Phytochrome nationallicence Lycopersicon nationallicence Photomorphogenic mutants nationallicence Photomorphogenesis nationallicence FR : far-red light nationallicence PHY : undesignated phytochrome gene nationallicence PHYA, PHYB , etc. : phytochrome gene of type A, B, etc nationallicence PHY : undesignated phytochrome apoprotein nationallicence PHYA, PHYB, etc. : phytochrome apoprotein of type A, B, etc nationallicence phy : undesignated holophytochrome nationallicence phyA, phyB, etc. : holophytochrome of type A, B, etc nationallicence R : red light nationallicence WL : white light nationallicence WT : wild type nationallicence Kerckhoffs L. Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703, BD Wageningen, The Netherlands aut Van Tuinen A. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, 6703, HA Wageningen, The Netherlands aut Hauser B. Botany Department, University of Georgia, 30602, Athens, GA, USA aut Cordonnier-Pratt M.-M Botany Department, University of Georgia, 30602, Athens, GA, USA aut Nagatani A. Laboratory for Photoperception and Signal Transduction, Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, 351-01, Saitama, Japan aut Koornneef M. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, 6703, HA Wageningen, The Netherlands aut Pratt L. Botany Department, University of Georgia, 30602, Athens, GA, USA aut Kendrick R. Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703, BD Wageningen, The Netherlands aut Planta Springer-Verlag 199/1(1996-05-01), 152-157 0032-0935 199:1<152 1996 199 425 https://doi.org/10.1007/BF00196891 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196891 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kerckhoffs L. Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703, BD Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Van Tuinen A. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, 6703, HA Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Hauser B. Botany Department, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Cordonnier-Pratt M.-M Botany Department, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Nagatani A. Laboratory for Photoperception and Signal Transduction, Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, 351-01, Saitama, Japan aut NATIONALLICENCE 700 1- Koornneef M. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, 6703, HA Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Pratt L. Botany Department, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Kendrick R. Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703, BD Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 152-157 0032-0935 199:1<152 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer