caa a22 4500 477101291 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196876 doi (NATIONALLICENCE)springer-10.1007/BF00196876 Characterization of a calcium/calmodulin-dependent protein kinase homolog from maize roots showing light-regulated gravitropism [Elektronische Daten] [Ying-Tang Lu, Hiroyoshi Hidaka, Lewis Feldman] Roots of many species respond to gravity (gravitropism) and grow downward only if illuminated. This light-regulated root gravitropism is phytochrome-dependent, mediated by calcium, and inhibited by KN-93, a specific inhibitor of calcium/calmodulin-dependent protein kinase II (CaMK II). A cDNA encoding MCK1, a maize homolog of mammalian CaMK, has been isolated from roots of maize (Zea mays L.). The MCK1 gene is expressed in root tips, the site of perception for both light and gravity. Using the [35S]CaM gel-overlay assay we showed that calmodulin-binding activity of the MCK1 is abolished by 50 μM KN-93, but binding is not affected by 5 μM KN-93, paralleling physiological findings that light-regulated root gravitropism is inhibited by 50 μM KN-93, but not by 5 μM KN-93. KN-93 inhibits light-regulated gravitropism by interrupting transduction of the light signal, not light perception, suggesting that MCK1 may play a role in transducing light. This is the first report suggesting a physiological function for a CaMK homolog in light signal transduction. Springer-Verlag, 1996 Calmodulin nationallicence Light nationallicence Gravitropism nationallicence Protein kinase nationallicence Zea (gravitropism) nationallicence CaM : calmodulin nationallicence CaMK (II) : Ca2+/calmodulin-dependent protein kinase (II) nationallicence CBP : CaM-binding protein nationallicence CDPK : Ca2+-dependent protein kinase nationallicence MCK1 : maize homolog of mamalian CaMK nationallicence Lu Ying-Tang Department of Plant Biology, University of California, 94720, Berkeley, CA, USA aut Hidaka Hiroyoshi Department of Pharmacology, Nagoya University School of Medicine, Nagoya, Japan aut Feldman Lewis Department of Plant Biology, University of California, 94720, Berkeley, CA, USA aut Planta Springer-Verlag 199/1(1996-05-01), 18-24 0032-0935 199:1<18 1996 199 425 https://doi.org/10.1007/BF00196876 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196876 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Ying-Tang Department of Plant Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Hidaka Hiroyoshi Department of Pharmacology, Nagoya University School of Medicine, Nagoya, Japan aut NATIONALLICENCE 700 1- Feldman Lewis Department of Plant Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 18-24 0032-0935 199:1<18 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer