caa a22 4500 477101313 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196892 doi (NATIONALLICENCE)springer-10.1007/BF00196892 Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents [Elektronische Daten] Size and shape of intracellular oil bodies are determined by the oleosins/oils ratio [Julie Ting, Keunmyoung Lee, Chandra Ratnayake, Kathryn Platt, Ronald Balsamo, Anthony Huang] In seeds, the subcellular storage oil bodies have a matrix of oils (triacylglycerols) surrounded by a layer of phospholipids embedded with abundant structural proteins called oleosins. We used two maize (Zea mays L.) strains having diverse kernel (seed) oil contents to study the effects of varying the oil and oleosin contents on the structure of the oil bodies. Illinois High Oils (IHO, 15% w/w oils) and Illinois Low Oils (ILO, 0.5%) maize kernels were the products of breeding for diverse oil contents for about 100 generations. In both maize strains, although the genes for oil synthesis had apparently been modified drastically, the genes encoding oleosins appeared to be unaltered, as revealed by Southern blot analyses of the three oleosin genes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting of the oleosins. In addition, both strains contained the same three oleosin isoforms of a defined proportion, and both accumulated oils and oleosins coordinately. Oleosins in both strains were restricted to the oil bodies, as shown by analyses of the various subcellular fractions separated by sucrosedensity-gradient centrifugation. Electron microscopy of the embryos and the isolated organelles revealed that the oil bodies in IHO were larger and had a spherical shape, whereas those in ILO were smaller and had irregular shapes. We conclude that in seeds, oleosin genes are expressed independent of the oil contents, and the size and shape of the oil bodies are dictated by the ratio of oils to oleosins synthesized during seed maturation. The extensive breeding for diverse oil contents has not altered the apparent mechanism of oil-body synthesis and the occurrence of hetero-dimer or -multimer of oleosin isoforms on the oil bodies. Springer-Verlag, 1996 Illinois High Oils nationallicence Illinois Low Oils nationallicence Oil body nationallicence Oleosin nationallicence Oleosin gene nationallicence Zea (oil body) nationallicence IHO : Illinois High Oils nationallicence ILO : Illinois Low Oils nationallicence Ting Julie Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Lee Keunmyoung Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Ratnayake Chandra Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Platt Kathryn Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Balsamo Ronald Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Huang Anthony Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Planta Springer-Verlag 199/1(1996-05-01), 158-165 0032-0935 199:1<158 1996 199 425 https://doi.org/10.1007/BF00196892 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196892 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ting Julie Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Lee Keunmyoung Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Ratnayake Chandra Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Platt Kathryn Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Balsamo Ronald Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Huang Anthony Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 158-165 0032-0935 199:1<158 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer