caa a22 4500 477101321 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196880 doi (NATIONALLICENCE)springer-10.1007/BF00196880 Overexpression of rice phytochrome A in tobacco seedlings modifies responsiveness but not competence to phytochrome [Elektronische Daten] [Karlheinz Emmler, Thomas Kretsch, Eberhard Schäfer] To analyse the control of rice phytochrome A (phyA) overexpression (wild type or variously mutated) on gene regulation, transgenic tobacco lines overexpressing various rice phyA constructs were crossed with transgenic tobacco lines containing mustard Lhcb1 or Chs1 promoters fused to the uidA reporter gene (β-glucuronidase). It was demonstrated that the temporal pattern of competence to respond to phytochrome was not altered by rice phyA overexpression. Also, overexpression of rice phyA did not change the spatial pattern of gene expression. The responsiveness to red and far-red light, on the other hand, depended on the type of overexpressed rice phyA in a structure-function relation: the serine-to-alanine mutant mediated an enhanced response both under continuous red and far-red light, whereas the N-terminal deletion mutant showed a dominant negative effect under continuous far-red light and even after red light pulses. However, the effectiveness of rice phyA overexpression depended on the promoter construct and the developmental stage of the seedlings. The Lhcb1 promoter also conferred β-glucuronidase activity in etiolated seedlings. This dark expression could be decreased by a long-wavelength farred light pulse given early in development (24 h after sowing), indicating that this phenomenon is under the control of stable types of phytochrome. Springer-Verlag, 1996 Chs1 (promoter) nationallicence GUS expression nationallicence Lhcb1 (promoter) nationallicence Nicotiana nationallicence phyA overexpression nationallicence Structure-function relation nationallicence Chs1 : chalcone synthase nationallicence GUS : β-glucuronidase nationallicence Lhcb1 : type 1 light-harvesting chlorophyll a/b-binding protein nationallicence NTD : N-terminal deletion mutant of rice phyA nationallicence phyA : phytochrome A nationallicence phyB : phytochrome B nationallicence Pfr : far-red absorbing form of phytochrome nationallicence Pr : red-absorbing form of phytochrome nationallicence RW : rice wild-type phyA nationallicence S/A : serine-to-alanine mutant of rice phyA nationallicence XAN : wild-type tobacco cv. Xanthi nationallicence Emmler Karlheinz Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany aut Kretsch Thomas Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany aut Schäfer Eberhard Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany aut Planta Springer-Verlag 199/1(1996-05-01), 50-56 0032-0935 199:1<50 1996 199 425 https://doi.org/10.1007/BF00196880 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196880 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Emmler Karlheinz Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany aut NATIONALLICENCE 700 1- Kretsch Thomas Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany aut NATIONALLICENCE 700 1- Schäfer Eberhard Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 50-56 0032-0935 199:1<50 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer