caa a22 4500 477101348 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196882 doi (NATIONALLICENCE)springer-10.1007/BF00196882 Glucan phosphorylases in Vicia faba L.: cloning, structural analysis and expression patterns of cytosolic and plastidic forms in relation to starch [Elektronische Daten] [Peter Buchner, Ljudmilla Borisjuk, Ulrich Wobus] We have isolated and characterised cDNA sequences from a Vicia faba cotyledonary library encoding a plastidic isoform (VfPhol) and a cytosolic isoform (VfPho2) of an α-1,4-glucan phosphorylase (EC 2.4.1.1; Commission on Plant Gene Nomenclature 1994). The Phol isoform is characterized by the presence of a plastidial transit peptide and an 81-residue stretch of additional amino acids in the middle of the polypeptide which are not found in the Pho2 isoform. We define the position of this so-called insertion sequence differently from previous authors. The Phol transcripts were found predominantly in the early seed coat and in cotyledons, and accumulated until the late desiccation phase, whereas Pho2 transcripts were about equally abundant in all investigated tissues. Activity patterns of both enzymes in cotyledons roughly followed mRNA accumulation patterns, with the exception of the late desiccation phases when mRNAs were degraded but enzyme activities remained at high level, even in long-stored seeds. The distinct Phol and Pho2 gene expression patterns in seed coats coincided with the transient accumulation pattern of starch. Similarly, in-situ hybridisation revealed a relationship between Phol gene expression and starch granule formation in developing cotyledons. Expression data and enzyme activity patterns were associated with starch formation during seed development, and could simply reflect a continuous accumulation of enzyme protein, ensuring immediate participation in starch degradation during germination. Springer-Verlag, 1996 α-1,4-Glucan phosphorylase (Phol and Pho2 isoforms) nationallicence Differential gene expression nationallicence Seed development nationallicence Starch synthesis nationallicence Starch degradation nationallicence Vicia nationallicence DAF : days after fertilisation nationallicence RACE : rapid amplification of cDNA ends nationallicence RT-PCR : reverse transcriptase-polymerase chain reaction nationallicence VfPhol : plastidic glucan phosphorylase of Vicia faba L nationallicence VfPho2 : cytosolic glucan phosphorylase of Vicia faba L nationallicence Buchner Peter Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466, Gatersleben, Germany aut Borisjuk Ljudmilla Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466, Gatersleben, Germany aut Wobus Ulrich Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466, Gatersleben, Germany aut Planta Springer-Verlag 199/1(1996-05-01), 64-73 0032-0935 199:1<64 1996 199 425 https://doi.org/10.1007/BF00196882 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196882 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buchner Peter Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Borisjuk Ljudmilla Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Wobus Ulrich Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466, Gatersleben, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 64-73 0032-0935 199:1<64 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer