caa a22 4500 477101402 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196888 doi (NATIONALLICENCE)springer-10.1007/BF00196888 Long-term drought stress induces structural and functional reorganization of photosystem II [Elektronische Daten] [M. Giardi, A. Cona, B. Geiken, T. Kučera, J. Masojídek, A. Mattoo] Long-term drought stress on photosystem II (PSII) was studied in pea (Pisum sativum L.) seedlings. Drought stress (reduction of water content by 35-80%) led to a considerable depletion of the PSII core, and the remaining PSII complex appeared to be functional and reorganized, with a unit size (LHCP/PSII core) twofold greater than that of well-irrigated plants. By immunoblotting analysis of the PSII proteins from grana and stroma lamellae, the enhanced degradation of CP43 and D1 proteins was observed in water-stressed plants. Also, water stress caused increased phosphorylation of the PSII core and increased D1 protein synthesis. Water-stress-mediated increase in D1 synthesis did not occur when plants were exposed to photoinhibitory light. The depletion of the PSII core was essentially reversed when water-stressed plants grown at low visible irradiance were watered. We suggest that the syndrome caused by the effect of long-term water stress on photosynthesis is a combination of at least two events: a reduction in the number of active PSII centres caused by a physical destabilization of the PSII core and a PSII reorganization with enhanced D1 turnover to counteract the core depletion. Springer-Verlag, 1996 D1 protein (turnover, modification) nationallicence Drought nationallicence High irradiance nationallicence Photosystem II (core phosphorylation) nationallicence Pisum sativum (drought stress) nationallicence Stress syndrome nationallicence Chl : chlorophyll nationallicence CP43 and CP47 : β-carotene-Chla-proteins of PSII core nationallicence DCPIP : 2,6-dichlorophenolindophenol nationallicence DPC : diphenylcarbazide nationallicence Fv/Fm : the ratio of yield of variable fluorescence to yield of maximal fluorescence when all reaction centres are closed nationallicence LHC(P) : light-harvesting complex (proteins) nationallicence Wc : water content nationallicence Giardi M. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut Cona A. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut Geiken B. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut Kučera T. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut Masojídek J. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut Mattoo A. Plant Molecular Biology Laboratory, U.S. Department of Agriculture, BARC, 20705, Beltsville, MD, USA aut Planta Springer-Verlag 199/1(1996-05-01), 118-125 0032-0935 199:1<118 1996 199 425 https://doi.org/10.1007/BF00196888 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196888 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giardi M. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut NATIONALLICENCE 700 1- Cona A. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut NATIONALLICENCE 700 1- Geiken B. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut NATIONALLICENCE 700 1- Kučera T. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut NATIONALLICENCE 700 1- Masojídek J. Italian National Council of Research (BEV-CNR), Area della Ricerca di Roma, Via Salaria km 29.3, I-00016, Monterotondo Scalo, Italy aut NATIONALLICENCE 700 1- Mattoo A. Plant Molecular Biology Laboratory, U.S. Department of Agriculture, BARC, 20705, Beltsville, MD, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 118-125 0032-0935 199:1<118 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer