caa a22 4500 477101429 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196883 doi (NATIONALLICENCE)springer-10.1007/BF00196883 Nitrate assimilation during the anaerobic germination of rice: expression of ferredoxin-dependent glutamate synthase [Elektronische Daten] [Monica Mattana, Immacolata Coraggio, Ida Brambilla, Alcide Bertani, Remo Reggiani] Ferredoxin-dependent glutamate synthase (Fd-GOGAT; EC 1.4.7.1) is the last enzyme involved in the pathway of nitrate assimilation in higher plants. This paper describes the synthesis and expression of the enzyme in anaerobic coleoptiles of rice (Oryza sativa L.) and its regulation by exogenous nitrate. The activity of Fd-GOGAT was strongly inhibited by cycloheximide between 4 and 9 d of anaerobic germination. The addition of nitrate slightly increased, in the first 5 h, the specific activity of Fd-GOGAT as well as the amount of a 160-kDa protein specifically immunoprecipitated with anti-Fd-GOGAT serum. Northern blot analysis, performed with a specific riboprobe, showed the presence of mRNA of the expected size and the inductive effect of nitrate. The role of Fd-GOGAT is discussed in relation to the anaerobic assimilation of nitrate by rice coleoptiles. Springer-Verlag, 1996 Anoxia nationallicence Coleoptile nationallicence Glutamate synthase nationallicence Nitrate nationallicence Oryza nationallicence CHX : cycloheximide nationallicence Fd : ferredoxin nationallicence GOGAT : glutamate synthase nationallicence GS : glutamine synthetase nationallicence NiR : nitrite reductase nationallicence NR : nitrate reductase nationallicence Mattana Monica Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut Coraggio Immacolata Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut Brambilla Ida Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut Bertani Alcide Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut Reggiani Remo Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut Planta Springer-Verlag 199/1(1996-05-01), 74-78 0032-0935 199:1<74 1996 199 425 https://doi.org/10.1007/BF00196883 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196883 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mattana Monica Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut NATIONALLICENCE 700 1- Coraggio Immacolata Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut NATIONALLICENCE 700 1- Brambilla Ida Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut NATIONALLICENCE 700 1- Bertani Alcide Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut NATIONALLICENCE 700 1- Reggiani Remo Istituto Biosintesi Vegetali, C.N.R., via Bassini 15, I-20133, Milano, Italy aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 74-78 0032-0935 199:1<74 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer