caa a22 4500 477101453 CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196890 doi (NATIONALLICENCE)springer-10.1007/BF00196890 Subcellular localization of ubiquitin in plant protoplasts and the function of ubiquitin in selective degradation of outer-wall plasmodesmata in regenerating protoplasts [Elektronische Daten] [Katrin Ehlers, Margot Schulz, Rainer Kollmann] Immunocytochemical localizations in Vicia faba L. protoplasts and cultures of regenerating Solanum nigrum L. protoplasts support former observations that in plant cells ubiquitin occurs within the cytoplasm, the nucleus, the chloroplasts and at the plasmalemma, but not within the vacuole or the cell wall. Immunoresponses were also observed within mitochondria and associated with the endoplasmic reticulum, which is in accordance with previous findings on animal cells. Moreover, the tonoplast membrane system was found to be labelled. For regenerating S. nigrum protoplasts, evidence is given that ubiquitin plays a role in selective degradation even of whole subcellular structures. Most of the discontinuous plasmodesmata formed in the newly deposited outer cell walls during the early stages of culture disappear later on, except for those near the periphery of division walls or of non-division walls, which are probably used for the formation of continuous cell connections during further culture. Outer-wall plasmodesmata which are destined to disappear show high immunoreactivity to ubiquitin antibody, but no conspicuous immunolabelling was observed with the remaining plasmodesmata. Thus, the selective disintegration of whole plasmodesmatal structures is obviously regulated by ubiquitination of plasmodesmatal proteins. A model for the mechanism of degradation of outer-wall plasmodesmata during extension growth of the cell wall is presented. Springer-Verlag, 1996 Immunolocalization nationallicence Plasmodesmata degradation (outer cell wall) nationallicence Protoplast culture nationallicence Solanum nationallicence Ubiquitin nationallicence Vicia nationallicence Ehlers Katrin Botanisches Institut der Universität Kiel, Olshausenstrasse 40, D-24098, Kiel, Germany aut Schulz Margot Institut für Landwirtschaftliche Botanik der Universität Bonn, Meckenheimer Allee 176, D-53115, Bonn, Germany aut Kollmann Rainer Botanisches Institut der Universität Kiel, Olshausenstrasse 40, D-24098, Kiel, Germany aut Planta Springer-Verlag 199/1(1996-05-01), 139-151 0032-0935 199:1<139 1996 199 425 https://doi.org/10.1007/BF00196890 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196890 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ehlers Katrin Botanisches Institut der Universität Kiel, Olshausenstrasse 40, D-24098, Kiel, Germany aut NATIONALLICENCE 700 1- Schulz Margot Institut für Landwirtschaftliche Botanik der Universität Bonn, Meckenheimer Allee 176, D-53115, Bonn, Germany aut NATIONALLICENCE 700 1- Kollmann Rainer Botanisches Institut der Universität Kiel, Olshausenstrasse 40, D-24098, Kiel, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 139-151 0032-0935 199:1<139 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer