caa a22 4500 47710147X CHVBK 20180405111542.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00196878 doi (NATIONALLICENCE)springer-10.1007/BF00196878 Changes in D1-protein turnover and recovery of photosystem II activity precede accumulation of chlorophyll in plants after release from mineral stress [Elektronische Daten] [Heidrun Dannehl, Helga Wietoska, Heike Heckmann, Doris Godde] After seven weeks of a combined magnesium and sulphur deficiency, spinach (Spinacea oleracea L.) plants showed a substantial accumulation of inactivated photosystem II (PSII) centres as indicated by a 40% decrease of the chlorophyll (Chl) fluorescence parameter Fv/Fm (Fv being the yield of variable fluorescence and Fm the yield of maximal fluorescence when all reaction centres are closed) together with a severe loss of leaf Chl content of 75%. The responses of the photosynthetic apparatus were examined when the deficient plants were transferred back to a rich nutrient medium. During the first 24 h of the recovery phase, thylakoid protein synthesis measured as incorporation of [14C]leucine per unit of Chl increased substantially. The synthesis rate of the D1 reaction-centre polypeptide of PSII, which in the deficient plants was reduced to 50% of the non-deficient control, was stimulated eight- to ninefold. D1-protein content, which in the deficient plants was reduced to 40% of the non-deficient control, started to increase 2 d later. Thus, D1-protein degradation was also enhanced. The increased D1-protein turnover led to a rapid repair of the existing PSII centres as indicated by the rise of Fv/Fm. It was completed at day 7 of the recovery phase. At day 2 of the recovery phase, the synthesis of other thylakoid proteins such as the D2 protein, cytochrome b 559, CP 47 and the 33-kDa polypeptide of the water-splitting system, became stimulated. This process resulted in an accumulation of new PSII centres. During the first week, formation of new PSII centres was not associated with an increase in leaf Chl content. The Chl content of the recovering leaves only started to increase when the ratio of PSII polypeptides versus LHCII (light-harvesting complex of PSII), which was substantially diminished in the deficient plants, became comparable to that of the control. The recovery process was accompanied by substantial changes in thylakoid protein phosphorylation. Their relevance to thylakoid protein turnover and stability is discussed. Springer-Verlag, 1996 D1-protein turnover nationallicence Mineral stress nationallicence Photosystem II (recovery of activity) nationallicence Regreening nationallicence Spinacia nationallicence Chl : chlorophyll nationallicence cyt : cytochrome nationallicence Fo : yield of intrinsic fluorescence when all PSII centres are open in the dark nationallicence Fm : yield of maximal fluorescence when all reaction centres are closed nationallicence F′m : fluorescence yield when all reaction centres are closed (after a saturating flash) under steady-state conditions nationallicence Fv : yield of variable fluorescence, (difference between Foand Fm) nationallicence Δ F : yield of variable fluorescence under steady state conditions nationallicence LHC : light-harvesting complex nationallicence PQ : plastoquinone nationallicence QA : primary quinone acceptor of PSII nationallicence QB : secondary quinone acceptor of PSII nationallicence qP : photochemical quenching nationallicence qn : non-photochemical quenching nationallicence Dannehl Heidrun Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität, Geb. ND3, Universitätsstrasse 150, D-44780, Bochum, Germany aut Wietoska Helga Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität, Geb. ND3, Universitätsstrasse 150, D-44780, Bochum, Germany aut Heckmann Heike Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität, Geb. ND3, Universitätsstrasse 150, D-44780, Bochum, Germany aut Godde Doris Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität, Geb. ND3, Universitätsstrasse 150, D-44780, Bochum, Germany aut Planta Springer-Verlag 199/1(1996-05-01), 34-42 0032-0935 199:1<34 1996 199 425 https://doi.org/10.1007/BF00196878 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196878 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dannehl Heidrun Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität, Geb. ND3, Universitätsstrasse 150, D-44780, Bochum, Germany aut NATIONALLICENCE 700 1- Wietoska Helga Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität, Geb. ND3, Universitätsstrasse 150, D-44780, Bochum, Germany aut NATIONALLICENCE 700 1- Heckmann Heike Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität, Geb. ND3, Universitätsstrasse 150, D-44780, Bochum, Germany aut NATIONALLICENCE 700 1- Godde Doris Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität, Geb. ND3, Universitätsstrasse 150, D-44780, Bochum, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/1(1996-05-01), 34-42 0032-0935 199:1<34 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer