caa a22 4500 477101518 CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF00195733 doi (NATIONALLICENCE)springer-10.1007/BF00195733 The major birch pollen allergen, Bet v 1, shows ribonuclease activity [Elektronische Daten] [Albrecht Bufe, Michael Spangfort, Helga Kahlert, Max Schlaak, Wolf-Meinhard Becker] The major birch (Betula alba L.) pollen allergen, Bet v 1, has been shown to be homologous to pathogenesis-related proteins in a number of plants. Recently, it was demonstrated that a ginseng protein with high homology to an intracellular pathogenesis-related protein of parsley and to Bet v 1 is a ribonuclease (RNase). Birch pollen extract was separated in an RNase activity gel. Four major RNase bands were excised from the gel, reseparated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by Western blotting with a specific Bet v 1 monoclonal antibody and patient's serum. Thus the monomer and the dimer of Bet v 1 showed RNase activity. Purified recombinant Bet v 1 was shown to degrade plant RNA. The RNase activity of recombinant Bet v 1 was 180 units · mg−1. Springer-Verlag, 1996 Allergen nationallicence Betula (pollen) nationallicence Pathogenesis-related protein nationallicence Pollen nationallicence Ribonuclease nationallicence IPR : intracellular pathogenesis-related (protein) nationallicence PR : pathogenesis related nationallicence RNasin : RNase inhibitor nationallicence Bufe Albrecht Molecular Allergology, Research Center Borstel, Parkallee 22, D-23845, Borstel, Germany aut Spangfort Michael ALK Research, Horsholm, Denmark aut Kahlert Helga Research Laboratories, Allergopharma, D-21465, Reinbek, Germany aut Schlaak Max Molecular Allergology, Research Center Borstel, Parkallee 22, D-23845, Borstel, Germany aut Becker Wolf-Meinhard Molecular Allergology, Research Center Borstel, Parkallee 22, D-23845, Borstel, Germany aut Planta Springer-Verlag 199/3(1996-07-01), 413-415 0032-0935 199:3<413 1996 199 425 https://doi.org/10.1007/BF00195733 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195733 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bufe Albrecht Molecular Allergology, Research Center Borstel, Parkallee 22, D-23845, Borstel, Germany aut NATIONALLICENCE 700 1- Spangfort Michael ALK Research, Horsholm, Denmark aut NATIONALLICENCE 700 1- Kahlert Helga Research Laboratories, Allergopharma, D-21465, Reinbek, Germany aut NATIONALLICENCE 700 1- Schlaak Max Molecular Allergology, Research Center Borstel, Parkallee 22, D-23845, Borstel, Germany aut NATIONALLICENCE 700 1- Becker Wolf-Meinhard Molecular Allergology, Research Center Borstel, Parkallee 22, D-23845, Borstel, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/3(1996-07-01), 413-415 0032-0935 199:3<413 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer