caa a22 4500 477101542 CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF00195731 doi (NATIONALLICENCE)springer-10.1007/BF00195731 Ueno Osamu Laboratory of Developmental Physiology, Department of Applied Physiology, National Institute of Agrobiological Resources, Tsukuba, 305, Ibaraki, Japan aut Immunocytochemical localization of enzymes involved in the C3 and C4 pathways in the photosynthetic cells of an amphibious sedge, Eleocharis vivipara [Elektronische Daten] [Osamu Ueno] Eleocharis vivipara link, an amphibious leafless sedge, develops traits of C4 photosynthesis and Kranz anatomy in the terrestrial form but develops C3-like traits with non-Kranz anatomy when submerged. The cellular localization of C3 and C4 enzymes in the photosynthetic cells of the two forms was investigated by immunogold labeling and electron microscopy. The terrestrial form has mesophyll cells and three kinds of bundle sheath cell, namely, parenchyma sheath cells, non-chlorophyllous mestome sheath cells, and Kranz cells. Phosphoenol-pyruvate carboxylase (PEPCase) was present in the cytosol of both the mesophyll cells and the parenchyma sheath cells, with higher-density labeling in the latter, but not in the Kranz cells. Pyruvate, Pi dikinase (PPDK) was found at high levels in the chloroplasts of both the mesophyll cells and the parenchyma sheath cells with some-what stronger labeling in the latter. This enzyme was also absent from the Kranz cells. Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was found in the chloroplasts of all types of photosynthetic cell, but labeling was significantly less intense in the parenchyma sheath cells than in other types of cell. The submerged form also has three types of photosynthetic cell, as well as non-chlorophyllous mestome sheath cells, but it lacks the traits of Kranz anatomy as a consequence of modification of the cells. Rubisco was densely distributed in the chloroplasts of all the photosynthetic cells. However, PEPCase and PPDK were found in both the mesophyll cells and the parenchyma sheath cells but at lower levels than in the terrestrial form. These data reveal that the terrestrial form has a unique pattern of cellular localization of C3 and C4 enzymes, and they suggest that this pattern and the changes in the extent of accumulation of the various enzymes are the main factors responsible for the difference in photosynthetic traits between the two forms. Springer-Verlag, 1996 Amphibious plant nationallicence C3, C4 photosynthetic enzymes nationallicence Eleocharis (C3, C4 metabolism) nationallicence Enzyme immunocytochemical localization nationallicence CAM : crassulacean acid metabolism nationallicence MC : meso phyll cell nationallicence PSC : parenchyma sheath cell nationallicence KC : Kranz cell nationallicence PEP-Case : phosphoenolpyruvate carboxylase nationallicence PPDK : pyruvate, Pi dikinase nationallicence Rubisco : ribulose-1,5-bisphosphate carboxylase/oxygenase nationallicence LS : large subunit nationallicence RuBP : ribulose-1,5-bisphosphate nationallicence Planta Springer-Verlag 199/3(1996-07-01), 394-403 0032-0935 199:3<394 1996 199 425 https://doi.org/10.1007/BF00195731 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195731 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Ueno Osamu Laboratory of Developmental Physiology, Department of Applied Physiology, National Institute of Agrobiological Resources, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/3(1996-07-01), 394-403 0032-0935 199:3<394 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer