caa a22 4500 477101550 CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF00195742 doi (NATIONALLICENCE)springer-10.1007/BF00195742 Purification and characterization of hydroxycinnamoyl-coenzyme A: ω-hydroxypalmitic acid O -hydroxycinnamoyltransferase from tobacco ( Nicotiana tabacum L.) cell-suspension cultures [Elektronische Daten] [Samia Lotfy, Francine Javelle, Jonathan Negrel] An acyltransferase hydroxycinnamoyl-Coenzyme A: ω-hydroxypalmitic acid O-hydroxycinnamoyltransferase (HHT; EC 2.3.1.-), which transfers hydroxycinnamic acids from hydroxycinnamoyl-CoA thioesters to several hydroxylated fatty acid derivatives, was characterized from tobacco (Nicotiana tabacum L. cv. Xanthi nc) cell-suspension cultures. It exhibited the same properties as the enzyme previously detected in wound-healing potato tuber discs (Lotfy et al., 1994, Phytochemistry 35: 1419-1424), and especially a marked specificity for ω-hydroxypalmitic acid and feruloyl-CoA. It was purified 300-fold to near homogeneity from late logarithmic-phase cell suspensions. The apparent molecular mass of the native protein was 55 kDa and its isoelectric point, estimated by electrofocusing, was 4.6. The purified enzyme conjugated ferulic acid to ω-hydroxypalmitic acid and to 1-tetradecanol, its main lipidic substrates, suggesting that the same enzyme probably synthesizes the different esters of 1-alkanols and of ω-hydroxy fatty acids which are formed in vitro. Springer-Verlag, 1996 1-Alkanols nationallicence Cell suspension cultures (tobacco) nationallicence Hydroxycinnamoyltransferases nationallicence ω-Hydroxypalmitic acid nationallicence Nicotiana nationallicence ABA : abscisic acid nationallicence IEF : isoelectric focusing nationallicence HHT : hydroxycinnamoyl-Coenzyme A: ω-hydroxypalmitic acid O-hydroxycinnamoyltransferase nationallicence pI : isoelectric point nationallicence Lotfy Samia Station de Génétique et d'Amélioration des Plantes, Laboratoire de Phytoparasitologie INRA-CNRS, F-21034, Dijon cedex, France aut Javelle Francine Station de Génétique et d'Amélioration des Plantes, Laboratoire de Phytoparasitologie INRA-CNRS, F-21034, Dijon cedex, France aut Negrel Jonathan Station de Génétique et d'Amélioration des Plantes, Laboratoire de Phytoparasitologie INRA-CNRS, F-21034, Dijon cedex, France aut Planta Springer-Verlag 199/3(1996-07-01), 475-480 0032-0935 199:3<475 1996 199 425 https://doi.org/10.1007/BF00195742 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195742 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lotfy Samia Station de Génétique et d'Amélioration des Plantes, Laboratoire de Phytoparasitologie INRA-CNRS, F-21034, Dijon cedex, France aut NATIONALLICENCE 700 1- Javelle Francine Station de Génétique et d'Amélioration des Plantes, Laboratoire de Phytoparasitologie INRA-CNRS, F-21034, Dijon cedex, France aut NATIONALLICENCE 700 1- Negrel Jonathan Station de Génétique et d'Amélioration des Plantes, Laboratoire de Phytoparasitologie INRA-CNRS, F-21034, Dijon cedex, France aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/3(1996-07-01), 475-480 0032-0935 199:3<475 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer