caa a22 4500 477101569 CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF00195740 doi (NATIONALLICENCE)springer-10.1007/BF00195740 Expression of abundant mRNAs during somatic embryogenesis of white spruce [ Picea glauca (Moench) Voss] [Elektronische Daten] [Jin-Zhuo Dong, David Dunstan] Embryogenic tissues of white spruce [Picea glauca (Moench) Voss] remain in an early developmental stage while cultured on 2,4-dichlorophenoxyacetic acid and N6-benzyladenine, but develop to cotyledonary embryos when these phytohormones are replaced by abscisic acid. Twenty-eight cDNAs were isolated from cotyledonary embryos by differential screening against immature embryo and non-embryonic tissues. Temporal expression patterns of these cDNAs during ABA-stimulated somatic embryo development were observed. This showed that clones could be allocated to various groups, including embryo-abundant, embryo-maturation-induced, and those whose expression was modulated during embryo development, germination or in non-embryogenic tissues. Expression corresponding to these cDNA clones showed that there were various responses to exogenous ABA or polyethylene glycol during a period of 48 h. Analyses of DNA and predicted amino acid sequence revealed that 12 of 28 cDNA clones had no known homologues, while others were predicted to encode different late-embryogenesis-abundant proteins, early methionine-labelled proteins, storage proteins, heat-shock proteins, glycine-rich cell wall protein, metallothionein-like protein and some other metabolic enzymes. Springer-Verlag, 1996 Abscisic acid nationallicence Embryo abundant gene nationallicence Gene expression nationallicence Polyethylene glycol nationallicence Picea nationallicence Somatic embryogenesis nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence ABA : abscisic acid nationallicence BA : N6-benzyladenine nationallicence cDNA : complementary deoxyribonucleic acid nationallicence Em : early methionine-labelled nationallicence HSP : heat-shock protein nationallicence LEA : late embryogenesis abundant nationallicence PEG : polyethylene glycol nationallicence Dong Jin-Zhuo National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut Dunstan David National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut Planta Springer-Verlag 199/3(1996-07-01), 459-466 0032-0935 199:3<459 1996 199 425 https://doi.org/10.1007/BF00195740 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195740 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Jin-Zhuo National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Dunstan David National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/3(1996-07-01), 459-466 0032-0935 199:3<459 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer