caa a22 4500 477101615 CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF00195737 doi (NATIONALLICENCE)springer-10.1007/BF00195737 Role of ω-3 fatty acid desaturases in the regulation of the level of trienoic fatty acids during leaf cell maturation [Elektronische Daten] [Gorou Horiguchi, Hiroaki Kodama, Mitsuo Nishimura, Koh Iba] Trienoic fatty acids, namely α-linolenic acid and hexadecatrienoic acid, present in leaf lipids are produced by ω-3 fatty acid desaturases located in the endoplasmic reticulum and plastid membranes. The changes in the level of trienoic fatty acids during leaf maturation were investigated in wild-type plants of Arabidopsis thaliana (L.) Heynh. and in the fad7 mutant deficient in the activity of a plastid ω-3 desaturase. The levels of trienoic fatty acids increased in 26 °C- and 15 °C-grown wild-type plants with maturation of leaves. The increase in trienoic fatty acids was mainly due to galactolipids enriched in plastid membranes. In addition, the relative levels of trienoic fatty acids in major glycerolipids, including phospholipids enriched in the endoplasmic reticulum membranes, also increased with leaf maturation. By contrast, when the fad7 mutant was grown at 26 °C, the relative levels of trienoic fatty acids in individual lipids decreased with leaf maturation. The decreases in the levels of trienoic fatty acids, however, were alleviated when the fad7 mutant was grown at 15 °C. These results suggest that the plastid ω-3 desaturase plays a major role in increasing the levels of trienoic fatty acids with leaf maturation. Springer-Verlag, 1996 Arabidopsis nationallicence ω-3 fatty acid desaturase nationallicence Leaf cell maturity nationallicence Linolenic acid nationallicence Lipid transfer nationallicence Mutant nationallicence 16∶3 : hexadecatrienoic acid nationallicence 18∶3 : α-linolenic acid nationallicence DGD : digalactosyldiacylglycerol nationallicence MGD : monogalactosyldiacylglycerol nationallicence PC : phosphatidylcholine nationallicence PE : phosphatidylethanolamine nationallicence TA : trienoic fatty acid nationallicence WT : wild type nationallicence ω-3 : refers to the position of the double bond from the methyl end of a fatty acid nationallicence Horiguchi Gorou Department of Biology, Faculty of Science, Kyushu University, 33, Hakozaki, Higashi-ku, 812-81, Fukuoka, Japan aut Kodama Hiroaki Department of Biology, Faculty of Science, Kyushu University, 33, Hakozaki, Higashi-ku, 812-81, Fukuoka, Japan aut Nishimura Mitsuo Department of Biology, Faculty of Science, Kyushu University, 33, Hakozaki, Higashi-ku, 812-81, Fukuoka, Japan aut Iba Koh Department of Biology, Faculty of Science, Kyushu University, 33, Hakozaki, Higashi-ku, 812-81, Fukuoka, Japan aut Planta Springer-Verlag 199/3(1996-07-01), 439-442 0032-0935 199:3<439 1996 199 425 https://doi.org/10.1007/BF00195737 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195737 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Horiguchi Gorou Department of Biology, Faculty of Science, Kyushu University, 33, Hakozaki, Higashi-ku, 812-81, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kodama Hiroaki Department of Biology, Faculty of Science, Kyushu University, 33, Hakozaki, Higashi-ku, 812-81, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nishimura Mitsuo Department of Biology, Faculty of Science, Kyushu University, 33, Hakozaki, Higashi-ku, 812-81, Fukuoka, Japan aut NATIONALLICENCE 700 1- Iba Koh Department of Biology, Faculty of Science, Kyushu University, 33, Hakozaki, Higashi-ku, 812-81, Fukuoka, Japan aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/3(1996-07-01), 439-442 0032-0935 199:3<439 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer