caa a22 4500 47710164X CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF00195724 doi (NATIONALLICENCE)springer-10.1007/BF00195724 Changes in blue-light-dependent protein phosphorylation during the early development of etiolated oat seedlings [Elektronische Daten] [Michael Salomon, Markus Zacherl, Wolfhart Rüdiger] Blue light induced the phosphorylation of a 116-kDa plasma-membrane-associated protein in dark-grown seedlings from Avena sativa L. The response was restricted to the phototropically sensitive tissue of the coleoptile tip. Surprisingly, this protein showed different properties in membrane preparations from plants that were grown for 3 d than in those from 5-d-old seedlings. In contrast to the younger coleoptiles, in 5-d-old seedlings phosphorylation of the 116-kDa protein depended strictly on the addition of Triton X-100 or other non-ionic detergents and was not abolished when the membranes were pretreated with trypsin. These latter membranes were also characterized by the appearance of two additional bluelight-regulated phosphoproteins of slightly lower molecular masses, exhibiting properties similar to the 116-kDa protein from 3-d-old plants. The data, together with solubilization studies, indicate that the 116-kDa protein is strongly membrane-bound only at the very beginning of seedling development and becomes more loosely associated in the course of coleoptile growth. In addition, we demonstrate that the capacity of the light-activated photoreceptor to recover photosensitivity in the dark also can occur under in-vitro conditions. Springer-Verlag, 1996 Avena nationallicence Photoreceptor nationallicence Phototropism nationallicence Plasma membrane nationallicence Protein kinase nationallicence Protein phosphorylation (blue-light-dependent) nationallicence LM-proteins : lower molecular mass phosphoproteins, 100 kDa and 95 kDa nationallicence NPM : N-phenylmaleimide nationallicence Salomon Michael Botanisches Institut der Universität München, Menzinger Strasse 67, D-80638, München, Germany aut Zacherl Markus Botanisches Institut der Universität München, Menzinger Strasse 67, D-80638, München, Germany aut Rüdiger Wolfhart Botanisches Institut der Universität München, Menzinger Strasse 67, D-80638, München, Germany aut Planta Springer-Verlag 199/3(1996-07-01), 336-342 0032-0935 199:3<336 1996 199 425 https://doi.org/10.1007/BF00195724 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195724 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salomon Michael Botanisches Institut der Universität München, Menzinger Strasse 67, D-80638, München, Germany aut NATIONALLICENCE 700 1- Zacherl Markus Botanisches Institut der Universität München, Menzinger Strasse 67, D-80638, München, Germany aut NATIONALLICENCE 700 1- Rüdiger Wolfhart Botanisches Institut der Universität München, Menzinger Strasse 67, D-80638, München, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/3(1996-07-01), 336-342 0032-0935 199:3<336 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer