caa a22 4500 477101658 CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF00195734 doi (NATIONALLICENCE)springer-10.1007/BF00195734 Cytoplasmic acidification as an early phosphorylation-dependent response of tobacco cells to elicitors [Elektronische Daten] [Yves Mathieu, Danielle Lapous, Sébastien Thomine, Christiane Laurière, Jean Guern] Elicitor-induced cytoplasmic pH changes of tobacco (Nicotiana tabacum L. cv. Xanthi) cells grown in suspension cultures were explored under a variety of conditions by using a flexible technique based on the distribution of [14C] benzoic acid between the intracellular and extracellular compartments. Comparison of data obtained by this technique and by 31P-nuclear magnetic resonance spectrometry qualifies the benzoic acid distribution method as a convenient and reliable way to probe cytoplasmic pH variations. Various elicitors shown to induce several defense-related responses in tobacco cells, namely oligogalacturonides of degree of polymerization 7-20, pectolyase from Aspergillus japonicus, Phytophthora megasperma crude elicitors and purified cryptogein, triggered cytoplasmic acidifications differing in intensity and kinetics according to the signal molecule. In contrast, no changes in cytoplasmic protons and external pH were observed in cells treated with short galacturonide oligomers, or with soybean-specific hepta β-glucoside from P. megasperma, which are devoid of elicitor activity in tobacco cells. The oligogalacturonide-induced cytoplasmic acidification was inhibited by two structurally unrelated protein kinase inhibitors, staurosporine and 6-dimethylaminopurine, which both reduced the external alkalinization response to the elicitor. The protein phosphatase inhibitor calyculin A alone behaved as an elicitormimicking molecule in triggering cytoplasmic acidification, again associated with extracellular alkalinization. These results indicate that the increase in the cytoplasmic concentration of protons may be considered as a common early intracellular response of tobacco cells to elicitors, associated with the extracellular alkalinization response and controlled by protein phosphorylation. Springer-Verlag, 1996 Cytosolic pH nationallicence Elicitor nationallicence Nicotiana nationallicence Protein phosphorylation nationallicence BA(H) : benzoic acid (protonated form) nationallicence 6-DMAP : 6-dimethylaminopurine nationallicence DP : degree of polymerization nationallicence Mes : 2-(N-morpholino)ethanesulfonic acid nationallicence OG : oligogalacturonide nationallicence pHc : cytoplasmic pH nationallicence 31P-NMR : nuclear magnetic resonance spectroscopy of 31P atoms nationallicence Mathieu Yves Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut Lapous Danielle Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut Thomine Sébastien Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut Laurière Christiane Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut Guern Jean Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut Planta Springer-Verlag 199/3(1996-07-01), 416-424 0032-0935 199:3<416 1996 199 425 https://doi.org/10.1007/BF00195734 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195734 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mathieu Yves Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut NATIONALLICENCE 700 1- Lapous Danielle Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut NATIONALLICENCE 700 1- Thomine Sébastien Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut NATIONALLICENCE 700 1- Laurière Christiane Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut NATIONALLICENCE 700 1- Guern Jean Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F91198, Gif-sur-Yvette Cedex, France aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/3(1996-07-01), 416-424 0032-0935 199:3<416 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer